Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells
المؤلفون المشاركون
Thabrew, M. Ira
Tennekoon, Kamani H.
Samarakoon, Sameera R.
Ediriweera, Meran K.
Abeysinghe, Nuwanka K.
Pathiranage, Varuni P. C.
Mendis, Anuka S.
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-19
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Vernonia zeylanica, is a shrub endemic to Sri Lanka.
V.
zeylanica has been used in Sri Lankan traditional medicine for the treatment of various diseases and conditions.
The present study was designed to determine antiproliferative, apoptotic, autophagic, and antioxidant effects of vernolactone, isolated from V.
zeylanica, in human embryonal carcinoma cells (NTERA-2, a cancer stem cell model).
Antiproliferative effects of vernolactone in NTERA-2 cells and human peripheral blood mononuclear cells (control cells) were evaluated using the Sulforhodamine B (SRB) assay and WST-1 antiproliferative assays, respectively.
The antiproliferative effect of vernolactone was further investigated using the colony formation assay.
Effects of vernolactone on apoptosis were investigated by phase contrast light microscopic and fluorescence microscopic analysis, caspase 3/7 expression, and real-time PCR of apoptosis-associated genes p53 and Survivin.
The effect of vernolactone on NTERA-2 cell migration was monitored using the wound healing assay.
Effects of vernolactone on the expression of autophagy-related genes (LC3, Beclin 1, PI3K, Akt, and mTOR) were evaluated using real-time PCR.
2,2-Diphenyl-1-2,2-diphenyl-picrylhydrazyl (DPPH) radical scavenging assay, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and ferric reducing antioxidant power (FRAP) assays were also carried out to evaluate the antioxidant activity of vernolactone.
Overall results confirm that vernolactone can exert antiproliferative effects, induce apoptosis and autophagy, and decrease NTERA-2 cell migration in a dose- and time-dependent manner with a very small antioxidant property.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abeysinghe, Nuwanka K.& Thabrew, M. Ira& Samarakoon, Sameera R.& Ediriweera, Meran K.& Tennekoon, Kamani H.& Pathiranage, Varuni P. C.…[et al.]. 2019. Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1209379
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abeysinghe, Nuwanka K.…[et al.]. Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells. Stem Cells International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1209379
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abeysinghe, Nuwanka K.& Thabrew, M. Ira& Samarakoon, Sameera R.& Ediriweera, Meran K.& Tennekoon, Kamani H.& Pathiranage, Varuni P. C.…[et al.]. Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1209379
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1209379
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر