Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells
Joint Authors
Thabrew, M. Ira
Tennekoon, Kamani H.
Samarakoon, Sameera R.
Ediriweera, Meran K.
Abeysinghe, Nuwanka K.
Pathiranage, Varuni P. C.
Mendis, Anuka S.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-19
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Vernonia zeylanica, is a shrub endemic to Sri Lanka.
V.
zeylanica has been used in Sri Lankan traditional medicine for the treatment of various diseases and conditions.
The present study was designed to determine antiproliferative, apoptotic, autophagic, and antioxidant effects of vernolactone, isolated from V.
zeylanica, in human embryonal carcinoma cells (NTERA-2, a cancer stem cell model).
Antiproliferative effects of vernolactone in NTERA-2 cells and human peripheral blood mononuclear cells (control cells) were evaluated using the Sulforhodamine B (SRB) assay and WST-1 antiproliferative assays, respectively.
The antiproliferative effect of vernolactone was further investigated using the colony formation assay.
Effects of vernolactone on apoptosis were investigated by phase contrast light microscopic and fluorescence microscopic analysis, caspase 3/7 expression, and real-time PCR of apoptosis-associated genes p53 and Survivin.
The effect of vernolactone on NTERA-2 cell migration was monitored using the wound healing assay.
Effects of vernolactone on the expression of autophagy-related genes (LC3, Beclin 1, PI3K, Akt, and mTOR) were evaluated using real-time PCR.
2,2-Diphenyl-1-2,2-diphenyl-picrylhydrazyl (DPPH) radical scavenging assay, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and ferric reducing antioxidant power (FRAP) assays were also carried out to evaluate the antioxidant activity of vernolactone.
Overall results confirm that vernolactone can exert antiproliferative effects, induce apoptosis and autophagy, and decrease NTERA-2 cell migration in a dose- and time-dependent manner with a very small antioxidant property.
American Psychological Association (APA)
Abeysinghe, Nuwanka K.& Thabrew, M. Ira& Samarakoon, Sameera R.& Ediriweera, Meran K.& Tennekoon, Kamani H.& Pathiranage, Varuni P. C.…[et al.]. 2019. Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1209379
Modern Language Association (MLA)
Abeysinghe, Nuwanka K.…[et al.]. Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells. Stem Cells International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1209379
American Medical Association (AMA)
Abeysinghe, Nuwanka K.& Thabrew, M. Ira& Samarakoon, Sameera R.& Ediriweera, Meran K.& Tennekoon, Kamani H.& Pathiranage, Varuni P. C.…[et al.]. Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1209379
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209379