Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity
المؤلفون المشاركون
Carollo, Carlos Alexandre
Lima, Emerson S.
de Picoli Souza, Kely
Santos, Edson Lucas dos
Antunes, Kátia Avila
Schmitz, Wanderlei Onofre
Alfredo, Tamaeh Monteiro
dos Santos, Jéssica Maurino
da Silva Melo da Cunha, Janielle
Costa, Edna Márcia Almeida
Boleti, Ana Paula de Araújo
Silva, Denise Brentan da
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-28
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Doxorubicin (DOX) is an efficient chemotherapeutic agent, but its clinical application is limited by its cardiotoxicity associated with increased oxidative stress.
Thus, the combination of DOX and antioxidants has been encouraged.
In this study, we evaluated (I) the chemical composition and antioxidant capacity of aqueous extracts from Guazuma ulmifolia stem bark (GUEsb) and leaves (GUEl) in 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, 2,2′-azobis(2-amidinopropane) dihydrochloride- (AAPH-) or DOX-induced lipid peroxidation inhibition in human blood cells, and intracellular reactive oxygen species (ROS) quantification using the fluorescent probe dichloro-dihydro-fluorescein diacetate (DCFH-DA) in K562 erythroleukemia cells incubated with GUEsb and stimulated with hydrogen peroxide; (II) the viability of K562 cells and human leukocytes treated with GUEsb in the absence or presence of DOX using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; (III) the acute toxicity of GUEsb; and (IV) the cardioprotective effect of GUEsb in C57Bl/6 mice treated with DOX.
The chemical composition indicated the presence of flavan-3-ol derivatives and condensed tannins in GUEsb and glycosylated flavonoids in GUEl.
GUEsb and GUEl showed free-radical scavenging antioxidant activity, antihemolytic activity, and AAPH- as well as DOX-induced malondialdehyde content reduction in human erythrocytes.
Based on its higher antioxidant potential, GUEsb was selected and subsequently showed intracellular ROS reduction without impairing the chemotherapeutic activity of DOX in K562 cells or inducing leukocyte cell death, but protected them against DOX-induced cell death.
Yet, GUEsb did not show in vivo acute toxicity, and it prevented MDA generation in the cardiac tissue of DOX-treated mice, thus demonstrating its cardioprotective effect.
Taken together, the results show that GUEsb and GUEl are natural alternatives to treat diseases associated with oxidative stress and that, in particular, GUEsb may play an adjuvant role in DOX chemotherapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
dos Santos, Jéssica Maurino& Alfredo, Tamaeh Monteiro& Antunes, Kátia Avila& da Silva Melo da Cunha, Janielle& Costa, Edna Márcia Almeida& Lima, Emerson S.…[et al.]. 2018. Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1211133
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
dos Santos, Jéssica Maurino…[et al.]. Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1211133
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
dos Santos, Jéssica Maurino& Alfredo, Tamaeh Monteiro& Antunes, Kátia Avila& da Silva Melo da Cunha, Janielle& Costa, Edna Márcia Almeida& Lima, Emerson S.…[et al.]. Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1211133
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211133
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر