Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity
Joint Authors
Carollo, Carlos Alexandre
Lima, Emerson S.
de Picoli Souza, Kely
Santos, Edson Lucas dos
Antunes, Kátia Avila
Schmitz, Wanderlei Onofre
Alfredo, Tamaeh Monteiro
dos Santos, Jéssica Maurino
da Silva Melo da Cunha, Janielle
Costa, Edna Márcia Almeida
Boleti, Ana Paula de Araújo
Silva, Denise Brentan da
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-28
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Doxorubicin (DOX) is an efficient chemotherapeutic agent, but its clinical application is limited by its cardiotoxicity associated with increased oxidative stress.
Thus, the combination of DOX and antioxidants has been encouraged.
In this study, we evaluated (I) the chemical composition and antioxidant capacity of aqueous extracts from Guazuma ulmifolia stem bark (GUEsb) and leaves (GUEl) in 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, 2,2′-azobis(2-amidinopropane) dihydrochloride- (AAPH-) or DOX-induced lipid peroxidation inhibition in human blood cells, and intracellular reactive oxygen species (ROS) quantification using the fluorescent probe dichloro-dihydro-fluorescein diacetate (DCFH-DA) in K562 erythroleukemia cells incubated with GUEsb and stimulated with hydrogen peroxide; (II) the viability of K562 cells and human leukocytes treated with GUEsb in the absence or presence of DOX using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; (III) the acute toxicity of GUEsb; and (IV) the cardioprotective effect of GUEsb in C57Bl/6 mice treated with DOX.
The chemical composition indicated the presence of flavan-3-ol derivatives and condensed tannins in GUEsb and glycosylated flavonoids in GUEl.
GUEsb and GUEl showed free-radical scavenging antioxidant activity, antihemolytic activity, and AAPH- as well as DOX-induced malondialdehyde content reduction in human erythrocytes.
Based on its higher antioxidant potential, GUEsb was selected and subsequently showed intracellular ROS reduction without impairing the chemotherapeutic activity of DOX in K562 cells or inducing leukocyte cell death, but protected them against DOX-induced cell death.
Yet, GUEsb did not show in vivo acute toxicity, and it prevented MDA generation in the cardiac tissue of DOX-treated mice, thus demonstrating its cardioprotective effect.
Taken together, the results show that GUEsb and GUEl are natural alternatives to treat diseases associated with oxidative stress and that, in particular, GUEsb may play an adjuvant role in DOX chemotherapy.
American Psychological Association (APA)
dos Santos, Jéssica Maurino& Alfredo, Tamaeh Monteiro& Antunes, Kátia Avila& da Silva Melo da Cunha, Janielle& Costa, Edna Márcia Almeida& Lima, Emerson S.…[et al.]. 2018. Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1211133
Modern Language Association (MLA)
dos Santos, Jéssica Maurino…[et al.]. Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1211133
American Medical Association (AMA)
dos Santos, Jéssica Maurino& Alfredo, Tamaeh Monteiro& Antunes, Kátia Avila& da Silva Melo da Cunha, Janielle& Costa, Edna Márcia Almeida& Lima, Emerson S.…[et al.]. Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1211133
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211133