Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery
المؤلفون المشاركون
Bodega, Guillermo
Alique, Matilde
Bohórquez, Lourdes
Ciordia, Sergio
Mena, María C.
Ramírez, Manuel R.
Morán, Miriam
Magro, Luis
Puebla, Lilian
Arza, Elvira
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-05
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
In a previous study, we demonstrated that endothelial microvesicles (eMVs) have a well-developed enzymatic team involved in reactive oxygen species detoxification.
In the present paper, we demonstrate that eMVs can synthesize the reducing power (NAD(P)H) that nourishes this enzymatic team, especially those eMVs derived from senescent human umbilical vein endothelial cells.
Moreover, we have demonstrated that the molecules that nourish the enzymatic machinery involved in NAD(P)H synthesis are blood plasma metabolites: lactate, pyruvate, glucose, glycerol, and branched-chain amino acids.
Drastic biochemical changes are observed in senescent eMVs to optimize the synthesis of reducing power.
Mitochondrial activity is diminished and the glycolytic pathway is modified to increase the activity of the pentose phosphate pathway.
Different dehydrogenases involved in NADPH synthesis are also increased.
Functional experiments have demonstrated that eMVs can synthesize NADPH.
In addition, the existence of NADPH in eMVs was confirmed by mass spectrometry.
Multiphoton confocal microscopy images corroborate the synthesis of reducing power in eMVs.
In conclusion, our present and previous results demonstrate that eMVs can act as autonomous reactive oxygen species scavengers: they use blood metabolites to synthesize the NADPH that fuels their antioxidant machinery.
Moreover, senescent eMVs have a stronger reactive oxygen species scavenging capacity than young eMVs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bodega, Guillermo& Alique, Matilde& Bohórquez, Lourdes& Morán, Miriam& Magro, Luis& Puebla, Lilian…[et al.]. 2018. Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211192
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bodega, Guillermo…[et al.]. Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211192
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bodega, Guillermo& Alique, Matilde& Bohórquez, Lourdes& Morán, Miriam& Magro, Luis& Puebla, Lilian…[et al.]. Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211192
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211192
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر