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Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery
Joint Authors
Bodega, Guillermo
Alique, Matilde
Bohórquez, Lourdes
Ciordia, Sergio
Mena, María C.
Ramírez, Manuel R.
Morán, Miriam
Magro, Luis
Puebla, Lilian
Arza, Elvira
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In a previous study, we demonstrated that endothelial microvesicles (eMVs) have a well-developed enzymatic team involved in reactive oxygen species detoxification.
In the present paper, we demonstrate that eMVs can synthesize the reducing power (NAD(P)H) that nourishes this enzymatic team, especially those eMVs derived from senescent human umbilical vein endothelial cells.
Moreover, we have demonstrated that the molecules that nourish the enzymatic machinery involved in NAD(P)H synthesis are blood plasma metabolites: lactate, pyruvate, glucose, glycerol, and branched-chain amino acids.
Drastic biochemical changes are observed in senescent eMVs to optimize the synthesis of reducing power.
Mitochondrial activity is diminished and the glycolytic pathway is modified to increase the activity of the pentose phosphate pathway.
Different dehydrogenases involved in NADPH synthesis are also increased.
Functional experiments have demonstrated that eMVs can synthesize NADPH.
In addition, the existence of NADPH in eMVs was confirmed by mass spectrometry.
Multiphoton confocal microscopy images corroborate the synthesis of reducing power in eMVs.
In conclusion, our present and previous results demonstrate that eMVs can act as autonomous reactive oxygen species scavengers: they use blood metabolites to synthesize the NADPH that fuels their antioxidant machinery.
Moreover, senescent eMVs have a stronger reactive oxygen species scavenging capacity than young eMVs.
American Psychological Association (APA)
Bodega, Guillermo& Alique, Matilde& Bohórquez, Lourdes& Morán, Miriam& Magro, Luis& Puebla, Lilian…[et al.]. 2018. Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211192
Modern Language Association (MLA)
Bodega, Guillermo…[et al.]. Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211192
American Medical Association (AMA)
Bodega, Guillermo& Alique, Matilde& Bohórquez, Lourdes& Morán, Miriam& Magro, Luis& Puebla, Lilian…[et al.]. Young and Especially Senescent Endothelial Microvesicles Produce NADPH: The Fuel for Their Antioxidant Machinery. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211192
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211192