Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells

المؤلفون المشاركون

Wang, Jiahong
Zhang, Zhou
Gu, Yan-ting
Zhang, Yuanyuan
Ren, Shu
Liu, Zheng

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-15، 15ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-07-29

دولة النشر

مصر

عدد الصفحات

15

التخصصات الرئيسية

الأحياء

الملخص EN

Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) are key transmembrane proteins leading to reactive oxygen species (ROS) overproduction.

However, the detailed roles of NOXs in retinal pigment epithelial (RPE) cell metabolic stress induced by Earle’s balanced salt solution (EBSS) through starvation remain unclear.

In this study, we investigated what roles NOXs play in regard to calpain activity, endoplasmic stress (ER), autophagy, and apoptosis during metabolic stress in ARPE-19 cells.

We first found that EBSS induced an increase in NOX2, NOX4, p22phox, and NOX5 compared to NOX1.

Secondly, suppression of NOXs resulted in reduced ER stress and autophagy, decreased ROS generation, and alleviated cell apoptosis.

Thirdly, silencing of NOX4, NOX5, and p22phox resulted in reduced levels of cell damage.

However, silencing of NOX1 was unaffected.

Finally, taurine critically mediated NOXs in response to EBSS stress.

In conclusion, this study demonstrated for the first time that NOX oxidases are the upstream regulators of calpain-2, ER stress, autophagy, and apoptosis.

Furthermore, the protective effect of taurine is mediated by the reduction of NOX-derived ROS, leading to sequential suppression of calpain induction, ER stress, autophagy, and apoptosis.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zhang, Yuanyuan& Ren, Shu& Gu, Yan-ting& Wang, Jiahong& Liu, Zheng& Zhang, Zhou. 2018. Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1211454

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zhang, Yuanyuan…[et al.]. Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1211454

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zhang, Yuanyuan& Ren, Shu& Gu, Yan-ting& Wang, Jiahong& Liu, Zheng& Zhang, Zhou. Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1211454

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1211454