Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells
Joint Authors
Wang, Jiahong
Zhang, Zhou
Gu, Yan-ting
Zhang, Yuanyuan
Ren, Shu
Liu, Zheng
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-29
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) are key transmembrane proteins leading to reactive oxygen species (ROS) overproduction.
However, the detailed roles of NOXs in retinal pigment epithelial (RPE) cell metabolic stress induced by Earle’s balanced salt solution (EBSS) through starvation remain unclear.
In this study, we investigated what roles NOXs play in regard to calpain activity, endoplasmic stress (ER), autophagy, and apoptosis during metabolic stress in ARPE-19 cells.
We first found that EBSS induced an increase in NOX2, NOX4, p22phox, and NOX5 compared to NOX1.
Secondly, suppression of NOXs resulted in reduced ER stress and autophagy, decreased ROS generation, and alleviated cell apoptosis.
Thirdly, silencing of NOX4, NOX5, and p22phox resulted in reduced levels of cell damage.
However, silencing of NOX1 was unaffected.
Finally, taurine critically mediated NOXs in response to EBSS stress.
In conclusion, this study demonstrated for the first time that NOX oxidases are the upstream regulators of calpain-2, ER stress, autophagy, and apoptosis.
Furthermore, the protective effect of taurine is mediated by the reduction of NOX-derived ROS, leading to sequential suppression of calpain induction, ER stress, autophagy, and apoptosis.
American Psychological Association (APA)
Zhang, Yuanyuan& Ren, Shu& Gu, Yan-ting& Wang, Jiahong& Liu, Zheng& Zhang, Zhou. 2018. Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1211454
Modern Language Association (MLA)
Zhang, Yuanyuan…[et al.]. Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1211454
American Medical Association (AMA)
Zhang, Yuanyuan& Ren, Shu& Gu, Yan-ting& Wang, Jiahong& Liu, Zheng& Zhang, Zhou. Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1211454
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211454