Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line
المؤلفون المشاركون
Endharti, Agustina Tri
Purnamasari, Yeni
Primasari, Renata
Poeranto, Sri
Permana, Sofy
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-11
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Coelomic fluid of Lumbricus rubellus (CFL) has attracted interest due to its pharmacological properties, including antitumor effect.
Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents.
This study aims to investigate whether the combination of CFL and 5-fluorouracil could reduce FAK protein level and iCa2+ and enhance p21 level.
Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents.
After 24 hours of treatment, it was necessary to assess the percentage of apoptosis, FAK, and p21 protein expression by flow cytometry.
iCa2+ concentration was measured using immunofluorescence.
The combination therapy of CFL with 5-fluorouracil potently suppressed six treatment groups were included in this study.
HT-29 cell lines were cultured and divided into six groups: group 1 was treated with vehicle (negative control), groups 2-5 were treated with 5-fluorouracil, groups 3-5 were treated with either CFL 5, 10, or 20 µg/ml immediately after 5-fluorouracil, and group 6 was treated with CFL 20 µg/ml, the progression of colorectal cancer.
Combination of CFL and 5-fluorouracil significantly decreased FAK expression (p<0.05), iCa2+ (p<0.05), and increased p21 expression (p<0.05) in HT-29 cells.
Our results suggest that CFL has an anticancer potential in colorectal cancer when combined with 5-fluorouracil.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Endharti, Agustina Tri& Purnamasari, Yeni& Primasari, Renata& Poeranto, Sri& Permana, Sofy. 2019. Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line. The Scientific World Journal،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1211855
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Endharti, Agustina Tri…[et al.]. Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line. The Scientific World Journal No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1211855
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Endharti, Agustina Tri& Purnamasari, Yeni& Primasari, Renata& Poeranto, Sri& Permana, Sofy. Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line. The Scientific World Journal. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1211855
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211855
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر