Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line
Joint Authors
Endharti, Agustina Tri
Purnamasari, Yeni
Primasari, Renata
Poeranto, Sri
Permana, Sofy
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Coelomic fluid of Lumbricus rubellus (CFL) has attracted interest due to its pharmacological properties, including antitumor effect.
Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents.
This study aims to investigate whether the combination of CFL and 5-fluorouracil could reduce FAK protein level and iCa2+ and enhance p21 level.
Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents.
After 24 hours of treatment, it was necessary to assess the percentage of apoptosis, FAK, and p21 protein expression by flow cytometry.
iCa2+ concentration was measured using immunofluorescence.
The combination therapy of CFL with 5-fluorouracil potently suppressed six treatment groups were included in this study.
HT-29 cell lines were cultured and divided into six groups: group 1 was treated with vehicle (negative control), groups 2-5 were treated with 5-fluorouracil, groups 3-5 were treated with either CFL 5, 10, or 20 µg/ml immediately after 5-fluorouracil, and group 6 was treated with CFL 20 µg/ml, the progression of colorectal cancer.
Combination of CFL and 5-fluorouracil significantly decreased FAK expression (p<0.05), iCa2+ (p<0.05), and increased p21 expression (p<0.05) in HT-29 cells.
Our results suggest that CFL has an anticancer potential in colorectal cancer when combined with 5-fluorouracil.
American Psychological Association (APA)
Endharti, Agustina Tri& Purnamasari, Yeni& Primasari, Renata& Poeranto, Sri& Permana, Sofy. 2019. Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line. The Scientific World Journal،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1211855
Modern Language Association (MLA)
Endharti, Agustina Tri…[et al.]. Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line. The Scientific World Journal No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1211855
American Medical Association (AMA)
Endharti, Agustina Tri& Purnamasari, Yeni& Primasari, Renata& Poeranto, Sri& Permana, Sofy. Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line. The Scientific World Journal. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1211855
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211855