Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows
المؤلفون المشاركون
Sordillo, Lorraine M.
Strickland, Jaimie M.
Lyman, Doug
Herdt, Thomas H.
Buchweitz, John P.
المصدر
Veterinary Medicine International
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-05
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Concerns regarding excessive hepatic copper concentrations in dairy cows have increased.
The objective of this study was to determine the association of hepatic copper concentrations with evidence of liver disease.
Blood and liver samples were collected at the time of slaughter in cull dairy cows (n=100).
Liver samples were analyzed for copper using inductively coupled plasma mass spectrometry and crude fat using liquid-liquid extraction and gravimetry.
Serum samples were analyzed for glutamate dehydrogenase, γ-glutamyltransferase, sorbitol dehydrogenase, aspartate aminotransferase activities, and bile acid concentrations.
Liver samples were examined histologically for inflammation, fibrosis, and rhodanine staining.
Animals were stratified by hepatic copper concentration and samples in the highest and lowest quintiles (Q5 and Q1) were evaluated for oxidative stress.
Systemic indices of oxidative stress included serum reactive oxygen and nitrogen species (RONS) and total antioxidant potential (AOP).
Tissue-level oxidative stress was assessed by immunohistochemistry using 4-hydroxynonenal (4HNE) and 3-nitrotyrosine (3NIT) stains to score the relative abundance and distribution of oxidized lipid and protein products, respectively.
Mean hepatic copper concentration was 496.83 μg/g and median 469.72 μg/g and ranged from 70.56 to 1264.27 μg/g dry tissue.
No association was found between hepatic copper concentrations and clinicopathological or histological evidence of hepatic damage or dysfunction.
There was a significant increase in the amount of IHC staining of 4HNE and 3NIT in Q5 compared with Q1.
Moreover, the IHC staining mirrored the distribution of the copper-specific stain rhodanine.
These results demonstrate that cows with elevated hepatic copper concentrations had no evidence of active liver disease but had increased hepatic oxidative stress.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Strickland, Jaimie M.& Lyman, Doug& Sordillo, Lorraine M.& Herdt, Thomas H.& Buchweitz, John P.. 2019. Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows. Veterinary Medicine International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1211945
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Strickland, Jaimie M.…[et al.]. Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows. Veterinary Medicine International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1211945
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Strickland, Jaimie M.& Lyman, Doug& Sordillo, Lorraine M.& Herdt, Thomas H.& Buchweitz, John P.. Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows. Veterinary Medicine International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1211945
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1211945
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر