Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows
Joint Authors
Sordillo, Lorraine M.
Strickland, Jaimie M.
Lyman, Doug
Herdt, Thomas H.
Buchweitz, John P.
Source
Veterinary Medicine International
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Concerns regarding excessive hepatic copper concentrations in dairy cows have increased.
The objective of this study was to determine the association of hepatic copper concentrations with evidence of liver disease.
Blood and liver samples were collected at the time of slaughter in cull dairy cows (n=100).
Liver samples were analyzed for copper using inductively coupled plasma mass spectrometry and crude fat using liquid-liquid extraction and gravimetry.
Serum samples were analyzed for glutamate dehydrogenase, γ-glutamyltransferase, sorbitol dehydrogenase, aspartate aminotransferase activities, and bile acid concentrations.
Liver samples were examined histologically for inflammation, fibrosis, and rhodanine staining.
Animals were stratified by hepatic copper concentration and samples in the highest and lowest quintiles (Q5 and Q1) were evaluated for oxidative stress.
Systemic indices of oxidative stress included serum reactive oxygen and nitrogen species (RONS) and total antioxidant potential (AOP).
Tissue-level oxidative stress was assessed by immunohistochemistry using 4-hydroxynonenal (4HNE) and 3-nitrotyrosine (3NIT) stains to score the relative abundance and distribution of oxidized lipid and protein products, respectively.
Mean hepatic copper concentration was 496.83 μg/g and median 469.72 μg/g and ranged from 70.56 to 1264.27 μg/g dry tissue.
No association was found between hepatic copper concentrations and clinicopathological or histological evidence of hepatic damage or dysfunction.
There was a significant increase in the amount of IHC staining of 4HNE and 3NIT in Q5 compared with Q1.
Moreover, the IHC staining mirrored the distribution of the copper-specific stain rhodanine.
These results demonstrate that cows with elevated hepatic copper concentrations had no evidence of active liver disease but had increased hepatic oxidative stress.
American Psychological Association (APA)
Strickland, Jaimie M.& Lyman, Doug& Sordillo, Lorraine M.& Herdt, Thomas H.& Buchweitz, John P.. 2019. Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows. Veterinary Medicine International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1211945
Modern Language Association (MLA)
Strickland, Jaimie M.…[et al.]. Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows. Veterinary Medicine International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1211945
American Medical Association (AMA)
Strickland, Jaimie M.& Lyman, Doug& Sordillo, Lorraine M.& Herdt, Thomas H.& Buchweitz, John P.. Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows. Veterinary Medicine International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1211945
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211945