How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions
المؤلفون المشاركون
Stuart, Jeffrey A.
Fonseca, Joao
Moradi, Fereshteh
Cunningham, Cassandra
Seliman, Bishoy
Worsfold, Cydney R.
Dolan, Sarah
Abando, John
Maddalena, Lucas A.
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-30
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Most mammalian tissue cells experience oxygen partial pressures in vivo equivalent to 1–6% O2 (i.e., physioxia).
In standard cell culture, however, headspace O2 levels are usually not actively regulated and under these conditions are ~18%.
This drives hyperoxia in cell culture media that can affect a wide variety of cellular activities and may compromise the ability of in vitro models to reproduce in vivo biology.
Here, we review and discuss some specific O2-consuming organelles and enzymes, including mitochondria, NADPH oxidases, the transplasma membrane redox system, nitric oxide synthases, xanthine oxidase, and monoamine oxidase with respect to their sensitivities to O2 levels.
Many of these produce reactive oxygen and/or nitrogen species (ROS/RNS) as either primary end products or byproducts and are acutely sensitive to O2 levels in the range from 1% to 18%.
Interestingly, many of them are also transcriptional targets of hypoxia-inducible factors (HIFs) and chronic cell growth at physioxia versus 18% O2 may alter their expression.
Aquaporins, which facilitate hydrogen peroxide diffusion into and out of cells, are also regulated by HIFs, indicating that O2 levels may affect intercellular communication via hydrogen peroxide.
The O2 sensitivities of these important activities emphasize the importance of maintaining physioxia in culture.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Stuart, Jeffrey A.& Fonseca, Joao& Moradi, Fereshteh& Cunningham, Cassandra& Seliman, Bishoy& Worsfold, Cydney R.…[et al.]. 2018. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1212167
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Stuart, Jeffrey A.…[et al.]. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1212167
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Stuart, Jeffrey A.& Fonseca, Joao& Moradi, Fereshteh& Cunningham, Cassandra& Seliman, Bishoy& Worsfold, Cydney R.…[et al.]. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1212167
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1212167
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر