How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions
Joint Authors
Stuart, Jeffrey A.
Fonseca, Joao
Moradi, Fereshteh
Cunningham, Cassandra
Seliman, Bishoy
Worsfold, Cydney R.
Dolan, Sarah
Abando, John
Maddalena, Lucas A.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Most mammalian tissue cells experience oxygen partial pressures in vivo equivalent to 1–6% O2 (i.e., physioxia).
In standard cell culture, however, headspace O2 levels are usually not actively regulated and under these conditions are ~18%.
This drives hyperoxia in cell culture media that can affect a wide variety of cellular activities and may compromise the ability of in vitro models to reproduce in vivo biology.
Here, we review and discuss some specific O2-consuming organelles and enzymes, including mitochondria, NADPH oxidases, the transplasma membrane redox system, nitric oxide synthases, xanthine oxidase, and monoamine oxidase with respect to their sensitivities to O2 levels.
Many of these produce reactive oxygen and/or nitrogen species (ROS/RNS) as either primary end products or byproducts and are acutely sensitive to O2 levels in the range from 1% to 18%.
Interestingly, many of them are also transcriptional targets of hypoxia-inducible factors (HIFs) and chronic cell growth at physioxia versus 18% O2 may alter their expression.
Aquaporins, which facilitate hydrogen peroxide diffusion into and out of cells, are also regulated by HIFs, indicating that O2 levels may affect intercellular communication via hydrogen peroxide.
The O2 sensitivities of these important activities emphasize the importance of maintaining physioxia in culture.
American Psychological Association (APA)
Stuart, Jeffrey A.& Fonseca, Joao& Moradi, Fereshteh& Cunningham, Cassandra& Seliman, Bishoy& Worsfold, Cydney R.…[et al.]. 2018. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1212167
Modern Language Association (MLA)
Stuart, Jeffrey A.…[et al.]. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1212167
American Medical Association (AMA)
Stuart, Jeffrey A.& Fonseca, Joao& Moradi, Fereshteh& Cunningham, Cassandra& Seliman, Bishoy& Worsfold, Cydney R.…[et al.]. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1212167
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212167