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Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection
المؤلفون المشاركون
Li, Hui
Xie, Can-Mao
Xiao, Yonglong
Cai, Hourong
Luo, Yi-Feng
Wang, Yong-Sheng
Yang, Qing
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-27
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Pseudomonas aeruginosa (PA) is one of the most prevalent pathogens that cause nosocomial infection in critical patients.
However, the mechanisms underlying macrophage growth status and functional changes during PA infection are yet unknown.
In the present study, NADPH oxidase, gp91phox (NOX2) mediated macrophage to senescence in a PAO1 colony-dependent manner.
gp91phox might regulate the senescence process through mutual interaction with the NF-κB pathway.
During infection, the overexpression or downregulation of gp91phox in macrophage could affect the nuclear activity of NF-κB p65, while the downregulation of NF-κB p65 led to a suppressed expression of gp91phox.
Reactive oxygen species (ROS) served as the second messenger between both molecules as the ROS inhibitor, N-acetylcysteine (NAC), could partially restore these changes.
Consequently, the level of ROS and inflammatory cytokines, including IL-6 and TNFα, elevated during PAO1 infection, and their production altered as a result of the genetic manipulation of gp91phox and NF-κB p65, as well as NAC treatment.
Also, the senescent phenotypes, SA-β-gal staining and p16ink4a, changed after genetic manipulation with gp91phox and NF-κB p65 and NAC treatment.
The capacity of phagocytosis in macrophages was decreased during senescence.
In conclusion, PA directs the macrophage towards senescence, and senescent macrophages exhibit a decreased ability of phagocytosis.
This process of senescence was regulated by the interactions between NADPH oxidase gp91phox and NF-κB p65 via ROS as a second messenger.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Hui& Luo, Yi-Feng& Wang, Yong-Sheng& Yang, Qing& Xiao, Yonglong& Cai, Hourong…[et al.]. 2018. Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1212404
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Hui…[et al.]. Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1212404
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Hui& Luo, Yi-Feng& Wang, Yong-Sheng& Yang, Qing& Xiao, Yonglong& Cai, Hourong…[et al.]. Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1212404
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1212404
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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