Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection
Joint Authors
Li, Hui
Xie, Can-Mao
Xiao, Yonglong
Cai, Hourong
Luo, Yi-Feng
Wang, Yong-Sheng
Yang, Qing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Pseudomonas aeruginosa (PA) is one of the most prevalent pathogens that cause nosocomial infection in critical patients.
However, the mechanisms underlying macrophage growth status and functional changes during PA infection are yet unknown.
In the present study, NADPH oxidase, gp91phox (NOX2) mediated macrophage to senescence in a PAO1 colony-dependent manner.
gp91phox might regulate the senescence process through mutual interaction with the NF-κB pathway.
During infection, the overexpression or downregulation of gp91phox in macrophage could affect the nuclear activity of NF-κB p65, while the downregulation of NF-κB p65 led to a suppressed expression of gp91phox.
Reactive oxygen species (ROS) served as the second messenger between both molecules as the ROS inhibitor, N-acetylcysteine (NAC), could partially restore these changes.
Consequently, the level of ROS and inflammatory cytokines, including IL-6 and TNFα, elevated during PAO1 infection, and their production altered as a result of the genetic manipulation of gp91phox and NF-κB p65, as well as NAC treatment.
Also, the senescent phenotypes, SA-β-gal staining and p16ink4a, changed after genetic manipulation with gp91phox and NF-κB p65 and NAC treatment.
The capacity of phagocytosis in macrophages was decreased during senescence.
In conclusion, PA directs the macrophage towards senescence, and senescent macrophages exhibit a decreased ability of phagocytosis.
This process of senescence was regulated by the interactions between NADPH oxidase gp91phox and NF-κB p65 via ROS as a second messenger.
American Psychological Association (APA)
Li, Hui& Luo, Yi-Feng& Wang, Yong-Sheng& Yang, Qing& Xiao, Yonglong& Cai, Hourong…[et al.]. 2018. Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1212404
Modern Language Association (MLA)
Li, Hui…[et al.]. Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1212404
American Medical Association (AMA)
Li, Hui& Luo, Yi-Feng& Wang, Yong-Sheng& Yang, Qing& Xiao, Yonglong& Cai, Hourong…[et al.]. Using ROS as a Second Messenger, NADPH Oxidase 2 Mediates Macrophage Senescence via Interaction with NF-κB during Pseudomonas aeruginosa Infection. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1212404
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212404