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Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human HematopoieticProgenitor Cells
المؤلفون المشاركون
Jeong, Jee-Yeong
Kang, Yun Gyeong
Lee, Tae-Hee
Lee, Ho Sup
Shin, Jung-Woog
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-27
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Ex vivo expansion of hematopoietic stem/progenitor cell (HSPC) has been investigated to improve the clinical outcome of HSPC transplantation.
However, ex vivo expansion of HSPCs still faces a major obstacle in that HPSCs tend to differentiate when proliferating.
Here, we cocultured HSPCs with mesenchymal stem cells (MSCs) and divided the HSPCs into two fractions according to whether they came into adherent to MSCs or not.
Additionally, we used hydrostatic pressure (HP) to mimic the physical conditions in vivo.
Even nonadherent cells expanded to yield a significantly larger number of total nucleated cells (TNCs), adherent cells maintained the HSPC phenotype (CD34+, CD34+CD38−, and CD133+CD38−) to a greater extent than nonadherent cells and had superior clonogenic potential.
Moreover, applying HP significantly increased the number of TNCs, the frequency of the immature HSPC phenotype, and the clonogenic potential.
Furthermore, the genetic markers for the HSPC niche were significantly increased under HP.
Our data suggest that the nonadherent fraction is the predominant site of HSPC expansion, whereas the adherent fraction seems to mimic the HSPC niche for immature cells.
Moreover, HP has a synergistic effect on expansion and functional maintenance.
This first study utilizing HP has a potential of designing clinically applicable expansion systems.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kang, Yun Gyeong& Jeong, Jee-Yeong& Lee, Tae-Hee& Lee, Ho Sup& Shin, Jung-Woog. 2018. Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human HematopoieticProgenitor Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213403
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kang, Yun Gyeong…[et al.]. Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human HematopoieticProgenitor Cells. Stem Cells International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1213403
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kang, Yun Gyeong& Jeong, Jee-Yeong& Lee, Tae-Hee& Lee, Ho Sup& Shin, Jung-Woog. Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human HematopoieticProgenitor Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213403
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1213403
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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