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Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human HematopoieticProgenitor Cells
Joint Authors
Jeong, Jee-Yeong
Kang, Yun Gyeong
Lee, Tae-Hee
Lee, Ho Sup
Shin, Jung-Woog
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-27
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Ex vivo expansion of hematopoietic stem/progenitor cell (HSPC) has been investigated to improve the clinical outcome of HSPC transplantation.
However, ex vivo expansion of HSPCs still faces a major obstacle in that HPSCs tend to differentiate when proliferating.
Here, we cocultured HSPCs with mesenchymal stem cells (MSCs) and divided the HSPCs into two fractions according to whether they came into adherent to MSCs or not.
Additionally, we used hydrostatic pressure (HP) to mimic the physical conditions in vivo.
Even nonadherent cells expanded to yield a significantly larger number of total nucleated cells (TNCs), adherent cells maintained the HSPC phenotype (CD34+, CD34+CD38−, and CD133+CD38−) to a greater extent than nonadherent cells and had superior clonogenic potential.
Moreover, applying HP significantly increased the number of TNCs, the frequency of the immature HSPC phenotype, and the clonogenic potential.
Furthermore, the genetic markers for the HSPC niche were significantly increased under HP.
Our data suggest that the nonadherent fraction is the predominant site of HSPC expansion, whereas the adherent fraction seems to mimic the HSPC niche for immature cells.
Moreover, HP has a synergistic effect on expansion and functional maintenance.
This first study utilizing HP has a potential of designing clinically applicable expansion systems.
American Psychological Association (APA)
Kang, Yun Gyeong& Jeong, Jee-Yeong& Lee, Tae-Hee& Lee, Ho Sup& Shin, Jung-Woog. 2018. Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human HematopoieticProgenitor Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213403
Modern Language Association (MLA)
Kang, Yun Gyeong…[et al.]. Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human HematopoieticProgenitor Cells. Stem Cells International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1213403
American Medical Association (AMA)
Kang, Yun Gyeong& Jeong, Jee-Yeong& Lee, Tae-Hee& Lee, Ho Sup& Shin, Jung-Woog. Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human HematopoieticProgenitor Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213403
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213403