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PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples
المؤلفون المشاركون
Sherwin, Virginia E.
Santi, Morena
Walker, Olivia
Pickwell, Natalie D.
Coffey, Tracey J.
Leigh, James A.
Egan, Sharon A.
المصدر
Veterinary Medicine International
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Streptococcus uberis is one of the leading causes worldwide of mastitis in the dairy industry, with the most likely sources of infection attributed to environmental reservoirs such as contaminated bedding materials.
Early detection of those cases most likely to progress to clinical disease would lead to improved animal welfare, a critical component of overall health and productivity.
A multiplex PCR-based diagnostic test was developed for detection of S.
uberis directly from milk and targeting two genes previously identified as important for intramammary colonisation and persistence in dairy cattle.
Results indicated the threshold for detection directly from milk was 20,000 CFU/ml and this was achieved without the need for preenrichment.
In addition, S.
uberis could be identified from milk samples collected during intramammary challenge studies, prior to clinical signs of infection and at much lower detection limits.
The PCR test developed for confirmation of the presence of S.
uberis directly from infected milk has potential value as a diagnostic test to identify early infection and/or to confirm that antibiotic therapy has been successful.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sherwin, Virginia E.& Santi, Morena& Walker, Olivia& Pickwell, Natalie D.& Coffey, Tracey J.& Leigh, James A.…[et al.]. 2020. PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples. Veterinary Medicine International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1214250
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sherwin, Virginia E.…[et al.]. PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples. Veterinary Medicine International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1214250
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sherwin, Virginia E.& Santi, Morena& Walker, Olivia& Pickwell, Natalie D.& Coffey, Tracey J.& Leigh, James A.…[et al.]. PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples. Veterinary Medicine International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1214250
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1214250
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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