PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples

Joint Authors

Sherwin, Virginia E.
Santi, Morena
Walker, Olivia
Pickwell, Natalie D.
Coffey, Tracey J.
Leigh, James A.
Egan, Sharon A.

Source

Veterinary Medicine International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-08

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Zoology

Abstract EN

Streptococcus uberis is one of the leading causes worldwide of mastitis in the dairy industry, with the most likely sources of infection attributed to environmental reservoirs such as contaminated bedding materials.

Early detection of those cases most likely to progress to clinical disease would lead to improved animal welfare, a critical component of overall health and productivity.

A multiplex PCR-based diagnostic test was developed for detection of S.

uberis directly from milk and targeting two genes previously identified as important for intramammary colonisation and persistence in dairy cattle.

Results indicated the threshold for detection directly from milk was 20,000 CFU/ml and this was achieved without the need for preenrichment.

In addition, S.

uberis could be identified from milk samples collected during intramammary challenge studies, prior to clinical signs of infection and at much lower detection limits.

The PCR test developed for confirmation of the presence of S.

uberis directly from infected milk has potential value as a diagnostic test to identify early infection and/or to confirm that antibiotic therapy has been successful.

American Psychological Association (APA)

Sherwin, Virginia E.& Santi, Morena& Walker, Olivia& Pickwell, Natalie D.& Coffey, Tracey J.& Leigh, James A.…[et al.]. 2020. PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples. Veterinary Medicine International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1214250

Modern Language Association (MLA)

Sherwin, Virginia E.…[et al.]. PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples. Veterinary Medicine International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1214250

American Medical Association (AMA)

Sherwin, Virginia E.& Santi, Morena& Walker, Olivia& Pickwell, Natalie D.& Coffey, Tracey J.& Leigh, James A.…[et al.]. PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples. Veterinary Medicine International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1214250

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1214250