Molecular localization of human papillomaviru_18 (HPV_18)‎ in tissues from thyroid carcinoma in mid-euphrates

الملخص EN

Background: THilnahyroid cancer is a cancer that starts in the thyroid gland and thought to be relatedtoanumberof environmental andgeneticpredisposingfactors .HumanPapil- loma Virus (HPV) the virus that causes cervical cancer ,is also linked to throat cancer and is one among theirassociatedinfectiveagents.

Objective: This study aimed at detecting DNA of HPV genotype-18 using in situ hybridization technique in thyroid tissues from benign thyroid hyperplasia and thyroid car- cinoma,andelucidatetheassociationbetweenthisHPV-18andthyroidcarcinogenesis.

Patients and Methods: Sixty (60) formalin-fixed, paraffin embedded thyroid tissue blocks were obtained ,among them (30) tissue biopsies from thyroid carcinoma with grade I and (20) benign thyroid neoplasm tissue blocks as well as (10) autopsies from apparently normal thyroid tissues were collected from the archives of Forensic Medicine Institute / Babil and used as thyroid healthy control group.

Detection of HPV-18 was done by using highly sensitive version of in situ hybridization technique.

Results: Among malignant thyroid tumors 56.7% patients had HPV-18 while 35% HPV- 18 positivity was detected in benign thyroid tumor group.

None of healthy thyroid tissues revealed ISH reactions.

There was 32.7% female had HPV-18, and 24% male had HPV-18.

Nosignificant statistical associations were noticed between the presence of HPV-18 and the age of those patients.

Conclusion: Human Papilloma-