Isolation and identification of pathogenic Pseudomonas aeruginosa from cow mastitis and detect some virulence factor using specific genetic marker

الملخص EN

This study performed to investigate distribution tox A و exo S gene among Pseudomonas aeruginosa isolated from milk in cows with mastitis using PCR technique.

82 sample from cow wound suffer from different region of Thi Qar city contaminated from period 12 july 2017-february 2018, Pseudomonas aeruginosa were isolated using cultured, microscopically and biochemical characteristics.

The result showed that 35 isolate blong to Pseudomonas aeruginosa these tested for their antibiotic sensitivity the isolate showed (100%) resistance to Ampicillin, oxacillin and (71.42%,57.14%,37.14%) resisitant to cefotamine, impenem, ticarcillin) respectively.

The ability of isolate to produce virulence factor also tested all isolate showed the ability to produce Haemolysin, phospholipase and only 54.28% of isolate can produce lecithinase and only 34.28% can produce alkaline protease.

PCR results showed that all 35(100 %) Pseudomonas aeruginosa was found carry the virulence gene TxoA and 19 (54.23%) found carry the virulence gene ExoS.