Isolation and identification of pathogenic Pseudomonas aeruginosa from cow mastitis and detect some virulence factor using specific genetic marker
Author
Source
Journal of College of Education for Pure Sciences
Issue
Vol. 11, Issue 1 (30 Jun. 2021), pp.1-9, 9 p.
Publisher
University of Thi-Qar College of Education for Pure Sciences
Publication Date
2021-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Economics & Business Administration
Abstract EN
This study performed to investigate distribution tox A و exo S gene among Pseudomonas aeruginosa isolated from milk in cows with mastitis using PCR technique.
82 sample from cow wound suffer from different region of Thi Qar city contaminated from period 12 july 2017-february 2018, Pseudomonas aeruginosa were isolated using cultured, microscopically and biochemical characteristics.
The result showed that 35 isolate blong to Pseudomonas aeruginosa these tested for their antibiotic sensitivity the isolate showed (100%) resistance to Ampicillin, oxacillin and (71.42%,57.14%,37.14%) resisitant to cefotamine, impenem, ticarcillin) respectively.
The ability of isolate to produce virulence factor also tested all isolate showed the ability to produce Haemolysin, phospholipase and only 54.28% of isolate can produce lecithinase and only 34.28% can produce alkaline protease.
PCR results showed that all 35(100 %) Pseudomonas aeruginosa was found carry the virulence gene TxoA and 19 (54.23%) found carry the virulence gene ExoS.
American Psychological Association (APA)
Sikhi, Rabab Jabbar. 2021. Isolation and identification of pathogenic Pseudomonas aeruginosa from cow mastitis and detect some virulence factor using specific genetic marker. Journal of College of Education for Pure Sciences،Vol. 11, no. 1, pp.1-9.
https://search.emarefa.net/detail/BIM-1382045
Modern Language Association (MLA)
Sikhi, Rabab Jabbar. Isolation and identification of pathogenic Pseudomonas aeruginosa from cow mastitis and detect some virulence factor using specific genetic marker. Journal of College of Education for Pure Sciences Vol. 11, no. 1 (Jun. 2021), pp.1-9.
https://search.emarefa.net/detail/BIM-1382045
American Medical Association (AMA)
Sikhi, Rabab Jabbar. Isolation and identification of pathogenic Pseudomonas aeruginosa from cow mastitis and detect some virulence factor using specific genetic marker. Journal of College of Education for Pure Sciences. 2021. Vol. 11, no. 1, pp.1-9.
https://search.emarefa.net/detail/BIM-1382045
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-9
Record ID
BIM-1382045