Hepatoprotective effect of pomegranate (punica granatum l)‎ peels powder against carbon tetrachloride (ccl4)‎-induced hepatotoxicity in rats

الملخص EN

Background: carbon tetrachloride (CCL4) is a colorless, volatile nonflammable liquid, a haloalkane and inhalation of its vapors can cause degeneration of the liver, kidneys and other organs through the generation of reactive oxidative stress.

pomegranate (punica granatum L) peel, a plant byproduct has been demonstrated to be a rich source of bioactive antioxidants mainly polyphenols which can inhibit and control hepatic injury with high efficacy and low toxicity.

aim of work: This study aimed to investigate the hepatoprotective impact of pomegranate peel powder (POP) on the hepatic toxicity caused by carbon tetrachloride.

methods: male albino rats (n = 30) were divided into 5 main groups (6 each).

as follows: group (1): negative group control fed in basal diet, Group (2): used as the positive control group (+ ve) and fed on basal diet.

Groups (3, 4 and 5) fed on a basal diet with POP at levels of 2, 4 and 6 g / kg / diet / day, respectively.

after, 29 days of the experiment, groups (2-5) were injected intraperitoneal with CCL4 (2 ml / b.w / rats to induce hepatic and other possible side effects as the renal disorders in rats, after 24 hours, rats were sacrificed and blood samples collected then centrifuged.

serum was kept frozen at-20°C until analysis.

results: results of this study demonstrated that POP at all levels considerably improved the CCl4-induced changes in hepatic function parameters (ALP, total protein, ALT, albumin, GGT, total bilirubin, AST, and globulin) and renal functions (creatinine, urea nitrogen and uric acid).

furthermore, POP administrations significantly diminished the elevation of serum TC, TG, VLDL-C and LDL-C values and elevated serum HDLC values.

in addition, it restored the activities of antioxidant enzymes SOD and GSH, which declined after CCl4 treatment and decreased MDA levels significantly.

conclusion: it could be concluded that pomegranate peel may guard against CCl4-induced hepatic injury through the organization of antioxidative activities.