Study on identification of five citrus sinensis cultivars based on similarity diversity in some genetic parameters of their molecular dna profiles using irap-pcr technique
العناوين الأخرى
دراسة على تعريف خمسة أصناف برتقال على اساس التشابهة والتباين في بعض القياسات الوراثيه لتهيئه جزيئات احماضها النوويه (دنا) باستخدام تقنيه PCR-IRAP
المؤلفون المشاركون
Sharaf, Muhammad Muhammad
Atawiah, Ahmad Rizq al-Sayyid
Hamdan, Nurah A.
al-Jaushi, Sharif Fathi
المصدر
Annals of Agricultural Science, Moshtohor
العدد
المجلد 60، العدد 1 (31 مارس/آذار 2022)، ص ص. 157-167، 11ص.
الناشر
تاريخ النشر
2022-03-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الموضوعات
الملخص EN
The present study was carried out on five sweet orange Cvs.
, namely White Khalili, Red Khalili, Succari, Navel and Mazizi to throw Their some light on genetic relationships and fingerprinting Profiles via 5 IRAP-PCR Primers.
These primers characterized by their higher to moderate degrees of successful in amplification potentiality for reproducible, polymorphic fragments, specific markers and Co-dominated, as well as their discriminatory power.
Obtained data regarding the genetic analysis of the 5 orange Cvs.
based on the 5 IRAP primers detected that 59 fragments were amplified from which 37 bands were polymorphic with 63% .
In addition, 29 bands of these poly morphic ones were positive unique and considered as specific markers.
So there 5 sweet orange Cvs didn't give identical DNA fingerprint.
The number of total and polymorphic amplified fragments across the 5 orange Cvs by each IRAP Primer showed a considerable variation.
Since, the F10&B6 was the superior (21 total and 18 polymorphic fragments), with the highest Polymorphism % (86).
In addition, the number of Positive unique DNA fragments (specific markers) generated by the F10&B6 primer varied greatly from one cultivar to another.
Herein Navel Orange cv.
occurred the highest number followed by Red Khalili, both (White Khalili & Succary) and finally Mazizi i.
e, exhibited 5, 4, 3, 3 and 2 specific markers, respectively.
Results of similarity matrix showed that the highest genetic Similarity (0.877) was existed between both Mazizi &White Khali.
Cvs, while the reverse was detected between White Khal.
& Red Khal.
(0, 716).
Beside, the UPĢMA dendrogram classified the 5 orange Cvs into two main groups (A &B) i.
e, (A.
) includes only Succari while (B) was subdivided into (C& D sub- groups) C includes White Khal.
only D included E & Fsub-sub clusters, E includes only Navel orange, while F (Mazizi &.
Red Khal.
).
So the IRAP- PCR an efficient technique for discriminating between Citrus sinensis cultivars even the closely related ones.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hamdan, Nurah A.& Sharaf, Muhammad Muhammad& Atawiah, Ahmad Rizq al-Sayyid& al-Jaushi, Sharif Fathi. 2022. Study on identification of five citrus sinensis cultivars based on similarity diversity in some genetic parameters of their molecular dna profiles using irap-pcr technique. Annals of Agricultural Science, Moshtohor،Vol. 60, no. 1, pp.157-167.
https://search.emarefa.net/detail/BIM-1390683
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sharaf, Muhammad Muhammad…[et al.]. Study on identification of five citrus sinensis cultivars based on similarity diversity in some genetic parameters of their molecular dna profiles using irap-pcr technique. Annals of Agricultural Science, Moshtohor Vol. 60, no. 1 (2022), pp.157-167.
https://search.emarefa.net/detail/BIM-1390683
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hamdan, Nurah A.& Sharaf, Muhammad Muhammad& Atawiah, Ahmad Rizq al-Sayyid& al-Jaushi, Sharif Fathi. Study on identification of five citrus sinensis cultivars based on similarity diversity in some genetic parameters of their molecular dna profiles using irap-pcr technique. Annals of Agricultural Science, Moshtohor. 2022. Vol. 60, no. 1, pp.157-167.
https://search.emarefa.net/detail/BIM-1390683
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
-
رقم السجل
BIM-1390683
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر