Study on identification of five citrus sinensis cultivars based on similarity diversity in some genetic parameters of their molecular dna profiles using irap-pcr technique

Abstract EN

The present study was carried out on five sweet orange Cvs.

, namely White Khalili, Red Khalili, Succari, Navel and Mazizi to throw Their some light on genetic relationships and fingerprinting Profiles via 5 IRAP-PCR Primers.

These primers characterized by their higher to moderate degrees of successful in amplification potentiality for reproducible, polymorphic fragments, specific markers and Co-dominated, as well as their discriminatory power.

Obtained data regarding the genetic analysis of the 5 orange Cvs.

based on the 5 IRAP primers detected that 59 fragments were amplified from which 37 bands were polymorphic with 63% .

In addition, 29 bands of these poly morphic ones were positive unique and considered as specific markers.

So there 5 sweet orange Cvs didn't give identical DNA fingerprint.

The number of total and polymorphic amplified fragments across the 5 orange Cvs by each IRAP Primer showed a considerable variation.

Since, the F10&B6 was the superior (21 total and 18 polymorphic fragments), with the highest Polymorphism % (86).

In addition, the number of Positive unique DNA fragments (specific markers) generated by the F10&B6 primer varied greatly from one cultivar to another.

Herein Navel Orange cv.

occurred the highest number followed by Red Khalili, both (White Khalili & Succary) and finally Mazizi i.

e, exhibited 5, 4, 3, 3 and 2 specific markers, respectively.

Results of similarity matrix showed that the highest genetic Similarity (0.877) was existed between both Mazizi &White Khali.

Cvs, while the reverse was detected between White Khal.

& Red Khal.

(0, 716).

Beside, the UPĢMA dendrogram classified the 5 orange Cvs into two main groups (A &B) i.

e, (A.

) includes only Succari while (B) was subdivided into (C& D sub- groups) C includes White Khal.

only D included E & Fsub-sub clusters, E includes only Navel orange, while F (Mazizi &.

Red Khal.

).

So the IRAP- PCR an efficient technique for discriminating between Citrus sinensis cultivars even the closely related ones.