Shogaol potentiates sevoflurane mediated neuroprotection against ischemia-reperfusion-induced brain injury via regulating apoptotic proteins and PI3K-Akt-mTOR-s6K signalling and HIF-1α-HO-1 expression

الملخص EN

The current research was intended to evaluate the impact of 6-shogaol in rodent model of ischemic-reperfusion induced- brain injury and also assessed 6-shogaol enhanced sevoflurane's neuroprotective effects.

Ischemic-Reperfusion (I/R) injury was induced by middle cerebral artery occlusion (MCAO) method in Sprague-Dawley rats.

A separate group of animal was exposed to sevoflurane (2.5%) post-conditioning for 1 h immediately after reperfusion.

The 6-shogaol (25 mg or 50 mg/kg body weight) was orally administered to treatment group rats for 14 days and then subjected to I/R.

The 6-shogaol treatment along with/without sevoflurane post-conditioning reduced the number of apoptotic cell counts, brain edema and cerebral infarct volume.

The western blotting analysis revealed a significant stimulation of the PI3K/Akt/mTOR signal pathway.

RT-PCR and western blotting studies revealed improved expressions of HIF-1α and HO-1 at both gene level and protein levels.

I/R induced neurological deficits were also alleviated on sevoflurane post-conditioning with/without 6-shogaol treatment.

The present findings revealed that pre-treatment with 6-shogoal enhanced the neuroprotective properties of sevoflurane post-conditioning, illustrated the efficacy of the compound against I/R injury.