In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells
المؤلفون المشاركون
Sasikumar, Raju
Das, Arub Jyoti
Deka, Sankar Chandra
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 28، العدد 9 (30 سبتمبر/أيلول 2021)، ص ص. 5338-5348، 11ص.
الناشر
تاريخ النشر
2021-09-30
دولة النشر
السعودية
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Cyanidin-3-glucoside (C3Ghv) compounds were purified and isolated from the anthocyanins extract of Haematocarpus validus.
C3Ghv were studied for antioxidant and cytoprotective properties on pancreatic β-cells of rat insulinoma cells (RINm5F) against the oxidative stress induced by streptozotocin (STZ).
The exposure of RINm5F cells to C3Ghv at concentration of 100 and 200 μg/mL for 24 h reduced 10% and 23% cell viability, respectively, as compared to control cells.
The pre-treatment of RINm5F cells with C3Ghv (50 µg/mL) increased the cell viability by 29% as compared to control, on being treated with STZ (10 mM) for 24 h.
The pre-treatment of RINm5F cells with C3Ghv (50 µg/mL) for 24 h followed by exposure to STZ (10 mM) for 1 h decreased the generation of reactive oxygen species (ROS) by 57%, generation of nitric oxide by 22.8%, generation of malondialdehyde (MDA) by 32%, the production of p-ERK ½ by 83%, p-JNK by 82.6%, p-MEK by 57%, and p-p38 MAPK by 64%.
The C3Ghv treatment also decreased the ratio of apoptotic proteins Bax to Bcl-2 by 61%, and improved the M2 phase of cell cycle by 75% as compared to STZ treated cells.
The overall results suggest that C3Ghv protects pancreatic β-cells against oxidative stress-induced apoptosis, thereby implicating the significant role of C3Ghv as an antidiabetic agent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sasikumar, Raju& Das, Arub Jyoti& Deka, Sankar Chandra. 2021. In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells. Saudi Journal of Biological Sciences،Vol. 28, no. 9, pp.5338-5348.
https://search.emarefa.net/detail/BIM-1411387
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sasikumar, Raju…[et al.]. In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells. Saudi Journal of Biological Sciences Vol. 28, no. 9 (2021), pp.5338-5348.
https://search.emarefa.net/detail/BIM-1411387
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sasikumar, Raju& Das, Arub Jyoti& Deka, Sankar Chandra. In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells. Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 9, pp.5338-5348.
https://search.emarefa.net/detail/BIM-1411387
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 5347-5348
رقم السجل
BIM-1411387
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر