Synergistic antitumor effect of thymoquinone combined with resveratrol against human lung cancer cells line
العناوين الأخرى
التأثير التآزري المضاد للأورام للثيموكينون مع الريسفيراترول ضد خط خلايا سرطان الرئة البشرية
المؤلف
al-Kharashi, Nuf Abd al-Karim Umar
المصدر
Journal of Medical and Pharmaceutical Sciences
العدد
المجلد 6، العدد 4 (30 سبتمبر/أيلول 2022)، ص ص. 25-41، 17ص.
الناشر
تاريخ النشر
2022-09-30
دولة النشر
فلسطين (قطاع غزة)
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Introduction: Thymoquinone (TQ) and resveratrol are natural anticancer and chemo-preventive agents through mechanism(s) that are not fully understood.
This study aimed to test the anticancer potential activity of the combination of TQ and Resveratrol, and their underlying mechanisms in the human lung cancer cell line, A549 cells.
Materials and methods: The A549 cells were treated with the different concentrations (25, 50, 75, 100, or 200 μM) of TQ, resveratrol, and their combination for 24h.
The antiproliferative activity against A549 cells was assessed using (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Trypan blue exclusion assay.
Apoptosis markers were assessed by using a real-time reverse transcriptase PCR Assay (rRT-PCR).
Results : We found that the proliferation of A549 lung cancer cells was inhibited by TQ, and resveratrol and their combination in a concentration-dependent manner, during a defined period.
The combination therapy also could inhibit the growth of A549 cells at 25 μM with a 50% inhibitory concentration (IC50) value of 37 ± 3.18μM.
These combinations were significantly (P < 0.001) elevated caspase-3, caspase-8, caspase-9, p53, p21, Fas, FasL, and Bax mRNA levels in treating A549 cells compared to the untreated cells.
The anticancer effect of this combination is mediated by the activation of the extrinsic and the intrinsic pathways.
Conclusion: all these results give a new promising combination with the enhanced anticancer effect.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Kharashi, Nuf Abd al-Karim Umar. 2022. Synergistic antitumor effect of thymoquinone combined with resveratrol against human lung cancer cells line. Journal of Medical and Pharmaceutical Sciences،Vol. 6, no. 4, pp.25-41.
https://search.emarefa.net/detail/BIM-1426376
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Kharashi, Nuf Abd al-Karim Umar. Synergistic antitumor effect of thymoquinone combined with resveratrol against human lung cancer cells line. Journal of Medical and Pharmaceutical Sciences Vol. 6, no. 4 (Sep. 2022), pp.25-41.
https://search.emarefa.net/detail/BIM-1426376
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Kharashi, Nuf Abd al-Karim Umar. Synergistic antitumor effect of thymoquinone combined with resveratrol against human lung cancer cells line. Journal of Medical and Pharmaceutical Sciences. 2022. Vol. 6, no. 4, pp.25-41.
https://search.emarefa.net/detail/BIM-1426376
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 37-41
رقم السجل
BIM-1426376
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر