Role of miR-214 in sensitization of human colorectal cancer cells to doxorubicin by p53 targeting

الملخص EN

Background: The upregulation of miR-214 is reported to reverse chemotherapeutic resistance or sensitivity in many human malignancies.

Objectives: This study aimed to investigate the potential function of miR-214 in the Apoptosis induction by targeting p53 in human Colorectal cancer cells (CRC) in combination with Doxorubicin (DOX).

Methods: miR-214 mimics were transfected to HT-29 CRC cells.

Following that, DOX was utilized to treat the transfected cells.

additionally, Apoptosis, migration, and cell viability were evaluated by flow cytometry, scratch-wound motility, and MTT assays, respectively.

Furthermore, qRT-PCR was employed to evaluate the expression level of miR-214 and p53.

Results: miR-214 transfection significantly inhibited the cell proliferation rate (P<0.

05), harnessed migration (P<0.

05), and induced Apoptosis (P<0.

05) in the HT-29 cells after 48 h.

Furthermore, more effectiveness was observed in combination with DOX.

Additionally, miR-214 transfection resulted in a reduction in p53 expression offering that might be a potential target for miR-214.

Conclusion: In conclusion, miR-214 sensitizes HT-29 cells to Doxorubicin by targeting p53 indicating the significant role of this miRNA in Colorectal cancer chemotherapy.