Role of miR-214 in sensitization of human colorectal cancer cells to doxorubicin by p53 targeting
Joint Authors
Wang, Li
Li, Xuefeng
Qu, Chenhui
Wei, Chunhong
Jiang, Peng
Wang, Lei
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 22, Issue 12 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Publication Date
2020-12-31
Country of Publication
United Arab Emirates
No. of Pages
6
Main Subjects
Abstract EN
Background: The upregulation of miR-214 is reported to reverse chemotherapeutic resistance or sensitivity in many human malignancies.
Objectives: This study aimed to investigate the potential function of miR-214 in the Apoptosis induction by targeting p53 in human Colorectal cancer cells (CRC) in combination with Doxorubicin (DOX).
Methods: miR-214 mimics were transfected to HT-29 CRC cells.
Following that, DOX was utilized to treat the transfected cells.
additionally, Apoptosis, migration, and cell viability were evaluated by flow cytometry, scratch-wound motility, and MTT assays, respectively.
Furthermore, qRT-PCR was employed to evaluate the expression level of miR-214 and p53.
Results: miR-214 transfection significantly inhibited the cell proliferation rate (P<0.
05), harnessed migration (P<0.
05), and induced Apoptosis (P<0.
05) in the HT-29 cells after 48 h.
Furthermore, more effectiveness was observed in combination with DOX.
Additionally, miR-214 transfection resulted in a reduction in p53 expression offering that might be a potential target for miR-214.
Conclusion: In conclusion, miR-214 sensitizes HT-29 cells to Doxorubicin by targeting p53 indicating the significant role of this miRNA in Colorectal cancer chemotherapy.
American Psychological Association (APA)
Wang, Li& Jiang, Peng& Qu, Chenhui& Li, Xuefeng& Wang, Lei& Wei, Chunhong. 2020. Role of miR-214 in sensitization of human colorectal cancer cells to doxorubicin by p53 targeting. Iranian Red Crescent Medical Journal،Vol. 22, no. 12, pp.1-6.
https://search.emarefa.net/detail/BIM-1430529
Modern Language Association (MLA)
Wang, Li…[et al.]. Role of miR-214 in sensitization of human colorectal cancer cells to doxorubicin by p53 targeting. Iranian Red Crescent Medical Journal Vol. 22, no. 12 (Dec. 2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1430529
American Medical Association (AMA)
Wang, Li& Jiang, Peng& Qu, Chenhui& Li, Xuefeng& Wang, Lei& Wei, Chunhong. Role of miR-214 in sensitization of human colorectal cancer cells to doxorubicin by p53 targeting. Iranian Red Crescent Medical Journal. 2020. Vol. 22, no. 12, pp.1-6.
https://search.emarefa.net/detail/BIM-1430529
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 5-6
Record ID
BIM-1430529