Detecting stx1 and stx2 genes from escherichia coli o157: h7 isolated from soft cheese samples collected from different district in Baghdad city-Iraq

الملخص EN

Escherichia coli O157: H7 colonies have been obtained from eighty produced local Buffalo's and Cow's soft cheese specimens that have been collected randomly from different retail markets in various districts of Baghdad city.

The results showed that the presence of Escherichia coli O157: H7 as a contamination indicator in the soft cheeses.

Six isolates were detected on the differential and selective media MacConkey agar, sorbitol such as MacConkey agar, chromogenic media and routine biochemical tests were used to diagnose and identify the bacteria.

Results of Escherichia coli O157: H7 diagnosis have been confirmed using PCR technique which exhibited that, 6 culturing strains serotype Escherichia coli O157: H7 were positive using PCR assay and 5 of these strains expressed gene of Stx1 while1strain expressed gene of Stx2.