Detecting stx1 and stx2 genes from escherichia coli o157: h7 isolated from soft cheese samples collected from different district in Baghdad city-Iraq
Other Title(s)
التحري عن Stx1 و Stx2 في بكتريا القولون H7: O157 المعزولة من عينات الجبن الطري المجموعة من مناطق مختلفة من بغداد-العراق
Joint Authors
Ali, Amal Husayn
Ismail, Lubna Iyyad
Kazim, Rima Jawad
Abd Allah, Taghrid Subayh
Source
Iraqi Journal of Science and Technology
Issue
Vol. 11, Issue 3 (30 Sep. 2020), pp.60-66, 7 p.
Publisher
Ministry of science and technology
Publication Date
2020-09-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract AR
عزلت مستعمرات ﺑﻜﺘﺮﯾﺎ القولون H7: O157 من 80 عينة من الجبن الطري المنتج محليا، مصدره حليب الأبقار و الجاموس و التي جمعت بشكل عشوائي من مختلف الأسواق في مواقع مختلفة من مدينة بغداد.
أظهرت النتائج وجود الاشريشيا القولونية O157: H كمؤشر للتلوث بهذه الأجبان الطرية.
تم الكشف عن ستة عزلات Escherichia coli O157:H7 على الأوساط التفاضلية و الانتقائية : وسط الماكونكي و السوربيتول ماكونكي اكار و الكروموجینیك و استخدمت الاختبارات الكيميائية الحيوية الروتينية لتشخيص و تحديد هذا النوع من البكتريا المعوية.
أكدت النتائج باستخدام تقنية ال PCR التي أظهرت عزل ستة أنواع من العزلات المصلية ﺑﻜﺘﺮﯾﺎ القولون O157:H7 خمسة من هذه العزلات تعبر عن جين Stx1 وعزلة واحدة تعبر عن جين Stx2.
Abstract EN
Escherichia coli O157: H7 colonies have been obtained from eighty produced local Buffalo's and Cow's soft cheese specimens that have been collected randomly from different retail markets in various districts of Baghdad city.
The results showed that the presence of Escherichia coli O157: H7 as a contamination indicator in the soft cheeses.
Six isolates were detected on the differential and selective media MacConkey agar, sorbitol such as MacConkey agar, chromogenic media and routine biochemical tests were used to diagnose and identify the bacteria.
Results of Escherichia coli O157: H7 diagnosis have been confirmed using PCR technique which exhibited that, 6 culturing strains serotype Escherichia coli O157: H7 were positive using PCR assay and 5 of these strains expressed gene of Stx1 while1strain expressed gene of Stx2.
American Psychological Association (APA)
Ali, Amal Husayn& Abd Allah, Taghrid Subayh& Ismail, Lubna Iyyad& Kazim, Rima Jawad. 2020. Detecting stx1 and stx2 genes from escherichia coli o157: h7 isolated from soft cheese samples collected from different district in Baghdad city-Iraq. Iraqi Journal of Science and Technology،Vol. 11, no. 3, pp.60-66.
https://search.emarefa.net/detail/BIM-1439822
Modern Language Association (MLA)
Ali, Amal Husayn…[et al.]. Detecting stx1 and stx2 genes from escherichia coli o157: h7 isolated from soft cheese samples collected from different district in Baghdad city-Iraq. Iraqi Journal of Science and Technology Vol. 11, no. 3 (2020), pp.60-66.
https://search.emarefa.net/detail/BIM-1439822
American Medical Association (AMA)
Ali, Amal Husayn& Abd Allah, Taghrid Subayh& Ismail, Lubna Iyyad& Kazim, Rima Jawad. Detecting stx1 and stx2 genes from escherichia coli o157: h7 isolated from soft cheese samples collected from different district in Baghdad city-Iraq. Iraqi Journal of Science and Technology. 2020. Vol. 11, no. 3, pp.60-66.
https://search.emarefa.net/detail/BIM-1439822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 64-66
Record ID
BIM-1439822
