Evaluation of Streptocin SH3, a Bacteriocin produced by streptococcus sanguinis isolated from human dental plaque
المؤلفون المشاركون
al-Musawi, Muna T.
al-Tai, Shahd Fadil
Rashid, Zayd S.
المصدر
al-Kindy College Medical Journal
العدد
المجلد 18، العدد 3 (31 ديسمبر/كانون الأول 2022)، ص ص. 201-206، 6ص.
الناشر
تاريخ النشر
2022-12-31
دولة النشر
العراق
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background: Bacteriocin is a peptidic toxin has many advantages to bacteria in their ecological niche and has strong antibacterial activity.
Objective: The aim of this study was to evaluation of bacteriocin using Streptococcus sanguinis isolated from human dental caries.
Subjects and Methods: Thirty five streptococcus isolates were diagnosed and tested for their production of bacteriocin, and then the optimal conditions for production of bacteriocin were determined.
After that, the purification of bacteriocin was made partially by ammonium sulfate at 95% saturation levels, followed by and gel filtration chromatography using Sephadex G-50 column.
Finally, physical characteristics were also studied and determined the bacteriocin stability.
Results: Among all streptococcal isolates, the S.
sanguinis SH3 isolate with batter antimicrobial activity was selected, and used in further experiments.
The optimum conditions for bacteriocin production were in Todd Hewitt broth (THB) supplemented with 1% glucose at 37°C for 24h under anaerobic conditions and inoculum size of (1.2 ˟ 109CFU/ml) and pH 7, where the inhibition zone diameter reached to 29 mm against Enterococcus faecalis.
The purified bacteriocin had high inhibitory activity against S.
pneumoniae, S.
pyogenes, E.
faecalis, E.
faecium and Leuconostoc mesenteroides.
Streptocin SH3 keeps its effectiveness within the pH range 3-10, and temperature until 80ºC for 20 min.
Conclusion: Bacteriocin produced from S.
sanguinis has high activity against many pathogens and has high stability over wide ranges of temperature and pH, which makes it a good alternative in the medical and food preservation fields
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Tai, Shahd Fadil& al-Musawi, Muna T.& Rashid, Zayd S.. 2022. Evaluation of Streptocin SH3, a Bacteriocin produced by streptococcus sanguinis isolated from human dental plaque. al-Kindy College Medical Journal،Vol. 18, no. 3, pp.201-206.
https://search.emarefa.net/detail/BIM-1492893
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Tai, Shahd Fadil…[et al.]. Evaluation of Streptocin SH3, a Bacteriocin produced by streptococcus sanguinis isolated from human dental plaque. al-Kindy College Medical Journal Vol. 18, no. 3 (Dec. 2022), pp.201-206.
https://search.emarefa.net/detail/BIM-1492893
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Tai, Shahd Fadil& al-Musawi, Muna T.& Rashid, Zayd S.. Evaluation of Streptocin SH3, a Bacteriocin produced by streptococcus sanguinis isolated from human dental plaque. al-Kindy College Medical Journal. 2022. Vol. 18, no. 3, pp.201-206.
https://search.emarefa.net/detail/BIM-1492893
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 205-206
رقم السجل
BIM-1492893
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر