Evaluation of Streptocin SH3, a Bacteriocin produced by streptococcus sanguinis isolated from human dental plaque
Joint Authors
al-Musawi, Muna T.
al-Tai, Shahd Fadil
Rashid, Zayd S.
Source
al-Kindy College Medical Journal
Issue
Vol. 18, Issue 3 (31 Dec. 2022), pp.201-206, 6 p.
Publisher
University of Baghdad al-Kindi College of Medicine
Publication Date
2022-12-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
Background: Bacteriocin is a peptidic toxin has many advantages to bacteria in their ecological niche and has strong antibacterial activity.
Objective: The aim of this study was to evaluation of bacteriocin using Streptococcus sanguinis isolated from human dental caries.
Subjects and Methods: Thirty five streptococcus isolates were diagnosed and tested for their production of bacteriocin, and then the optimal conditions for production of bacteriocin were determined.
After that, the purification of bacteriocin was made partially by ammonium sulfate at 95% saturation levels, followed by and gel filtration chromatography using Sephadex G-50 column.
Finally, physical characteristics were also studied and determined the bacteriocin stability.
Results: Among all streptococcal isolates, the S.
sanguinis SH3 isolate with batter antimicrobial activity was selected, and used in further experiments.
The optimum conditions for bacteriocin production were in Todd Hewitt broth (THB) supplemented with 1% glucose at 37°C for 24h under anaerobic conditions and inoculum size of (1.2 ˟ 109CFU/ml) and pH 7, where the inhibition zone diameter reached to 29 mm against Enterococcus faecalis.
The purified bacteriocin had high inhibitory activity against S.
pneumoniae, S.
pyogenes, E.
faecalis, E.
faecium and Leuconostoc mesenteroides.
Streptocin SH3 keeps its effectiveness within the pH range 3-10, and temperature until 80ºC for 20 min.
Conclusion: Bacteriocin produced from S.
sanguinis has high activity against many pathogens and has high stability over wide ranges of temperature and pH, which makes it a good alternative in the medical and food preservation fields
American Psychological Association (APA)
al-Tai, Shahd Fadil& al-Musawi, Muna T.& Rashid, Zayd S.. 2022. Evaluation of Streptocin SH3, a Bacteriocin produced by streptococcus sanguinis isolated from human dental plaque. al-Kindy College Medical Journal،Vol. 18, no. 3, pp.201-206.
https://search.emarefa.net/detail/BIM-1492893
Modern Language Association (MLA)
al-Tai, Shahd Fadil…[et al.]. Evaluation of Streptocin SH3, a Bacteriocin produced by streptococcus sanguinis isolated from human dental plaque. al-Kindy College Medical Journal Vol. 18, no. 3 (Dec. 2022), pp.201-206.
https://search.emarefa.net/detail/BIM-1492893
American Medical Association (AMA)
al-Tai, Shahd Fadil& al-Musawi, Muna T.& Rashid, Zayd S.. Evaluation of Streptocin SH3, a Bacteriocin produced by streptococcus sanguinis isolated from human dental plaque. al-Kindy College Medical Journal. 2022. Vol. 18, no. 3, pp.201-206.
https://search.emarefa.net/detail/BIM-1492893
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 205-206
Record ID
BIM-1492893