Reishi mushroom (Ganoderma lucidum)‎ intervention improves lipids profile and paraoxonase arylesterase activities in serum as well as enhances haemostatic effects in streptozotocin-induced diabetic rats

الملخص EN

Several strategies have been proposed to improve diabetes mellitus (DM) complications, because early treatment and prevention play a pivotal role in reducing the population burden of this disease.

The benefits of pharmaceutical agents for treating the disease have long been recommended, but medications may have unwanted side effects.

Therefore, the present study was carried out to investigate the potential effects of Ganoderma lucidum ethanol extract (GLE) intervention in modulating the hyperglycemia, serum lipids profile and serum paraoxonase/arylesterase activities, andhaemostatic effects in streptozotocin-induced diabetic rats.

Thirty-six male adult male Sprague-Dawley rats were randomly divided into equal six groups.

Group 1: Normal control, normal rats feed with basal diet (BD); Group 2: Model control, diabetic rats feed with BD without intervention; Groups 3-6: GLE, diabetic rats with BD, intervention groups utilizing GLE of 200, 400, 600 and 800 mg/kg bw by oral gavages for 28 consecutive days.

Type 2 diabetic rats were obtained by Streptozotocin (STZ) injection.

Treatment of rats with streptozotocin caused a significant increased (p < /em>≤0.05) in serum glucose, triglycerides (TG), total cholesterol (TC) and reactive oxygen species (ROS) concentrations by the ratio of 200.13, 554.05, 71.97 and 30.62% compared to normal controls, respectively.

The opposite direction was recorded for the high-density lipoprotein cholesterol (HDL-c) and antioxidant enzymes paraoxonase/arylesterase activities which decreased by the ratio of -57.95% , -50.81 and -41.32% compared to normal controls, respectively.

Additionally, haemostatic effects (bleeding and clotting times) were increased in different periods of times (7, 14, 21 and 28 days).

GLE intervention greatly ameliorated the hyperglycemia, serum lipid profiles, ROS and haemostatic effects in diabetic rats.

The rate of amelioration(s) was exhibited a dose- dependent increase with GLE intervention.

In conclusion, data of this study provided a basis for the use of GLE for the prevention and/or treatment of type-2 Diabetes mellitus complications such as hyperglycemia, oxidative stress and blood bleeding.