Molecular genetic fingerprint of some streptomyces isolated From Riyadh city

الملخص EN

The present investigation was conducted to develop molecular fingerprints for fifteen Streptomyces isolates gathered from Riyadh city by using RAPD-PCR.

Three 10-mer random primers (OPB-16, OPF-05 and OPK-10) out of eight had successfully generated reproducible polymorphic products.

Each of the three primers displayed a strong amplification with distinct bands.

The fingerprints generated by these primers revealed characteristic profiles for each of the fifteen Streptomyces isolate, in terms of number and position of RAPD bands.

Primer OPB-16 generates a total of 19 polymorphic bands.

Two of these polymorphic bands were identified as unique ones.

While the remaining ones were detected as 17 non unique bands.

The generated RAPD profile with the primer OPF-05 shows a total of 23 polymorphic bands among the studied isolates.

It comprises one unique band and 22 non unique polymorphic ones.

Similarly, primer OPK-10 develops 14 polymorphic bands which scored as 4 unique and 10 non unique bands.

The unique bands were used to discriminate between some of the studied Streptomyces isolates.

In addition, the other recorded polymorphic bands (non unique) provided a considerable number of combined class patterns that completly discrimination between all the studied isolates. The results generated from RAPD profiles were pooled for drawing the genetic relationships among the examined Streptomyces species.

The constructed dendrogram tree divided the fifteen isolates into two groups.

The highest similarity value (0.851) was observed between the two isolates no.

13 and 14.

On the other hand, the lowest similarity value (0.000) was scored between the two isolates no.

5 and 6.