Partial purification of acidic protease from the mung bean (pharsalus aurous roxby.)‎ seeds

الملخص EN

The purpose of the research was to study the extraction, purification and partial characterization of the protease from seeds of mung bean.

The enzyme was purified in a 2-step procedure involving ammonium sulphate at 90 % saturation, desalting by dialysis ; and ion exchange chromatography on a column containing DEAE-cellulose.

The activity of protease was higher at pH 6.

The optimum temperature of the enzyme was 40 °C.

The enzyme was show to have a higher specific activity (153 U / mg) and purified to 28 fold.