Partial purification of acidic protease from the mung bean (pharsalus aurous roxby.) seeds
Joint Authors
Merhij, Evan Ibrahim
al-Khafaji, Muhammad Abd Allah Jabr
Nimah, Ban Ahmad
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 17, Issue 2 (31 Aug. 2009), pp.620-624, 5 p.
Publisher
Publication Date
2009-08-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract AR
ان الغرض من هذه الدراسة هو استخلاص انزيم اليوريز من بذور نبات الماش و تنقية و توصيف جزئيا.
نقي الانزيم بخطوتين : اولا الترسيب بكبريتات الامونيوم بنسبة اشباع 90 % و الثانية طريقة التبادل الايوني باستخدام المبادل DEAE-cellulose.
اظهر الانزيم اعلى فعالية عند الرقم الهيدروجيني 6.
كما لوحظ ان اقصى فعالية للأنزيم كانت عند درجة حرارة 40 م.
اظهر الانزيم فعالية نوعية تقريبا 153 وحدة / ملغم بعدد مرات تنقية تقدر ب 28 مرة.
Abstract EN
The purpose of the research was to study the extraction, purification and partial characterization of the protease from seeds of mung bean.
The enzyme was purified in a 2-step procedure involving ammonium sulphate at 90 % saturation, desalting by dialysis ; and ion exchange chromatography on a column containing DEAE-cellulose.
The activity of protease was higher at pH 6.
The optimum temperature of the enzyme was 40 °C.
The enzyme was show to have a higher specific activity (153 U / mg) and purified to 28 fold.
American Psychological Association (APA)
al-Khafaji, Muhammad Abd Allah Jabr& Merhij, Evan Ibrahim& Nimah, Ban Ahmad. 2009. Partial purification of acidic protease from the mung bean (pharsalus aurous roxby.) seeds. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 17, no. 2, pp.620-624.
https://search.emarefa.net/detail/BIM-244635
Modern Language Association (MLA)
al-Khafaji, Muhammad Abd Allah Jabr…[et al.]. Partial purification of acidic protease from the mung bean (pharsalus aurous roxby.) seeds. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 17, no. 2 (2009), pp.620-624.
https://search.emarefa.net/detail/BIM-244635
American Medical Association (AMA)
al-Khafaji, Muhammad Abd Allah Jabr& Merhij, Evan Ibrahim& Nimah, Ban Ahmad. Partial purification of acidic protease from the mung bean (pharsalus aurous roxby.) seeds. Journal of Babylon University : Journal of Applied and Pure Sciences. 2009. Vol. 17, no. 2, pp.620-624.
https://search.emarefa.net/detail/BIM-244635
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 623-624
Record ID
BIM-244635
