Determination of catechol using flow- injection electrochemical detector and banana paste electrode : application to adrenaline determination

الملخص EN

This work involves indirect determination of catechol compounds using flow-injection electrochemical detector.

The detector based on the three-electrodes system, banana paste which contained the poly phenyl oxidase (PPO) enzyme as working electrode, auxiliary electrode Pt.

wire and silver/silver chloride, saturated potassium chloride Ag / AgCl .sat.

KCl as reference electrode.

The PPO enzyme in banana paste motivates the oxidation of the two hydroxyl group in catechol to the o-quinone which is then reduced at (-0.65) V back to catechol.

The reduction current was recorded and it is proportional to concentration of catechol.