Immobilization of urease in gelatin beads for urea estimation

الملخص EN

Urease was immobilized in gelatin beads.

The optimum immobilization (79 % activity) was observed at 4 C° with protein concentration of 1.0 mg / ml.

the apparent optimum pH shifted from 7.5 to 6.5.

Immobilized urease showed optimum activity at 60 C° compared with 40 C° for the soluble urease.

The immobilized urease remained very active over long period of time and this enzyme lost about 40 % of its original activity over the period of 60 days for storage at 4C compared with soluble urease which lost about 75 % its activity in the same conditions.

Blood urea estimation is carried out with immobilized enzyme beads compared with soluble enzyme and the beads can be used repeatedly for this purpose making it economical procedure compared to standard chemical method.