Immobilization of urease in gelatin beads for urea estimation
Joint Authors
Awad, Mufid Jalil
al-Khafaji, Muhammad Abd Allah Jabr
Source
Issue
Vol. 2009, Issue 33 (31 Mar. 2009), pp.131-137, 7 p.
Publisher
University of Babylon College of Sciences
Publication Date
2009-03-31
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract AR
تم تقيد أنزيم اليوريز باستخدام الجلاتين و كانت نسبة التقيد 79 % أظهر الأنزيم المقيد أعلى فعالية عند رقم هيدروجيني 6.5 مقارنة بالأنزيم الحر 7.5.
و أظهر أعلى فعالية عند درجة حرارة 60° م مقارنة بالأنزيم الحر (40° م).
بقي الأنزيم المقيد فعال لفترة طويلة حيث فقد حوالي 40 % من فعاليته عند خزنه لمدة 60 يوم مقارنة بالأنزيم الحر الذي فقد 75 % من فعاليته عند خزنه بنفس الظروف.
تم تقدير نسبة اليوريا بالدم باستخدام الأنزيم المقيد و كانت الطريقة كفؤة و اقتصادية.
Abstract EN
Urease was immobilized in gelatin beads.
The optimum immobilization (79 % activity) was observed at 4 C° with protein concentration of 1.0 mg / ml.
the apparent optimum pH shifted from 7.5 to 6.5.
Immobilized urease showed optimum activity at 60 C° compared with 40 C° for the soluble urease.
The immobilized urease remained very active over long period of time and this enzyme lost about 40 % of its original activity over the period of 60 days for storage at 4C compared with soluble urease which lost about 75 % its activity in the same conditions.
Blood urea estimation is carried out with immobilized enzyme beads compared with soluble enzyme and the beads can be used repeatedly for this purpose making it economical procedure compared to standard chemical method.
American Psychological Association (APA)
al-Khafaji, Muhammad Abd Allah Jabr& Awad, Mufid Jalil. 2009. Immobilization of urease in gelatin beads for urea estimation. National Journal of Chemistry،Vol. 2009, no. 33, pp.131-137.
https://search.emarefa.net/detail/BIM-254870
Modern Language Association (MLA)
al-Khafaji, Muhammad Abd Allah Jabr& Awad, Mufid Jalil. Immobilization of urease in gelatin beads for urea estimation. National Journal of Chemistry No. 33 (2009), pp.131-137.
https://search.emarefa.net/detail/BIM-254870
American Medical Association (AMA)
al-Khafaji, Muhammad Abd Allah Jabr& Awad, Mufid Jalil. Immobilization of urease in gelatin beads for urea estimation. National Journal of Chemistry. 2009. Vol. 2009, no. 33, pp.131-137.
https://search.emarefa.net/detail/BIM-254870
Data Type
Journal Articles
Language
English
Notes
20
Record ID
BIM-254870
