The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of fusobacterium spp. isolates from chronic periodontitis
المؤلف
المصدر
Journal of Baghdad College of Dentistry
العدد
المجلد 21، العدد 2 (30 يونيو/حزيران 2009)، ص ص. 87-90، 4ص.
الناشر
تاريخ النشر
2009-06-30
دولة النشر
العراق
عدد الصفحات
4
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : The microbial composition of dental plaque is highly diverse.
Fusobacterium spp.
is gram negative, nonmotile, anaerobic rods that are consider as part of the normal flora of oral cavity playing a major role in initiation & progression of periodontal diseases.
The purpose of the present study is to evaluate the plasmid stability & the association of the resistance gene with the plasmid in oral Fusobacterium spp.
Isolated from subjects with chronic periodontitis Materials and Method : A total of 11 Fusobacterium isolates were taken from 32 subjects with chronic periodontitis with average pocket depth range between 6- 8mm.
Antibiotic susceptibility to 12 antimicrobials, DNA extraction, plasmid profile analysis, curing, & conjugation & transformation were carried out on these isolates.
Results : The isolates showed variable degree of resistance to different antimicrobials but all were susceptible to Doxycycline.
Plasmid analysis of isolates, three major plasmid patterns were observed 3.1, 4.9 and 6.2-kb.
In curing experiments, resistance to Erythromycin, Penicillin, and Clarithromycin were lost together and their loss was accompanied by the loss of a plasmid of 3.1-kb.
Similarly, resistance was accompanied by the loss of a 6.2 –kb plasmid.
In transfer experiments, no resistance determinants were transferred by conjugation, indicating that they did not harbor conjugative plasmid.
Conclusion : The antimicrobial resistance among isolates is either plasmid- related for certain antibiotics or chromosomally- mediated for the others.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd al-Karim, Ali A.. 2009. The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of fusobacterium spp. isolates from chronic periodontitis. Journal of Baghdad College of Dentistry،Vol. 21, no. 2, pp.87-90.
https://search.emarefa.net/detail/BIM-288629
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abd al-Karim, Ali A.. The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of fusobacterium spp. isolates from chronic periodontitis. Journal of Baghdad College of Dentistry Vol. 21, no. 2 (2009), pp.87-90.
https://search.emarefa.net/detail/BIM-288629
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd al-Karim, Ali A.. The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of fusobacterium spp. isolates from chronic periodontitis. Journal of Baghdad College of Dentistry. 2009. Vol. 21, no. 2, pp.87-90.
https://search.emarefa.net/detail/BIM-288629
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 90
رقم السجل
BIM-288629
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر