![](/images/graphics-bg.png)
The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of fusobacterium spp. isolates from chronic periodontitis
Author
Source
Journal of Baghdad College of Dentistry
Issue
Vol. 21, Issue 2 (30 Jun. 2009), pp.87-90, 4 p.
Publisher
University of Baghdad College of Dentistry
Publication Date
2009-06-30
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Topics
Abstract EN
Background : The microbial composition of dental plaque is highly diverse.
Fusobacterium spp.
is gram negative, nonmotile, anaerobic rods that are consider as part of the normal flora of oral cavity playing a major role in initiation & progression of periodontal diseases.
The purpose of the present study is to evaluate the plasmid stability & the association of the resistance gene with the plasmid in oral Fusobacterium spp.
Isolated from subjects with chronic periodontitis Materials and Method : A total of 11 Fusobacterium isolates were taken from 32 subjects with chronic periodontitis with average pocket depth range between 6- 8mm.
Antibiotic susceptibility to 12 antimicrobials, DNA extraction, plasmid profile analysis, curing, & conjugation & transformation were carried out on these isolates.
Results : The isolates showed variable degree of resistance to different antimicrobials but all were susceptible to Doxycycline.
Plasmid analysis of isolates, three major plasmid patterns were observed 3.1, 4.9 and 6.2-kb.
In curing experiments, resistance to Erythromycin, Penicillin, and Clarithromycin were lost together and their loss was accompanied by the loss of a plasmid of 3.1-kb.
Similarly, resistance was accompanied by the loss of a 6.2 –kb plasmid.
In transfer experiments, no resistance determinants were transferred by conjugation, indicating that they did not harbor conjugative plasmid.
Conclusion : The antimicrobial resistance among isolates is either plasmid- related for certain antibiotics or chromosomally- mediated for the others.
American Psychological Association (APA)
Abd al-Karim, Ali A.. 2009. The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of fusobacterium spp. isolates from chronic periodontitis. Journal of Baghdad College of Dentistry،Vol. 21, no. 2, pp.87-90.
https://search.emarefa.net/detail/BIM-288629
Modern Language Association (MLA)
Abd al-Karim, Ali A.. The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of fusobacterium spp. isolates from chronic periodontitis. Journal of Baghdad College of Dentistry Vol. 21, no. 2 (2009), pp.87-90.
https://search.emarefa.net/detail/BIM-288629
American Medical Association (AMA)
Abd al-Karim, Ali A.. The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of fusobacterium spp. isolates from chronic periodontitis. Journal of Baghdad College of Dentistry. 2009. Vol. 21, no. 2, pp.87-90.
https://search.emarefa.net/detail/BIM-288629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 90
Record ID
BIM-288629