Isolation and molecular characterization of antibiotic producing bacteria from soil of al-Karak region and their potential as source of antimicrobial agents

العناوين الأخرى

عزل و دراسة الخصائص الجزيئية للبكتيريا المنتجة للمضادات الحيوية من التربة في منطقة الكرك و تأثيرها كمضادات بكتيرية

مقدم أطروحة جامعية

al-Tarawinah, Ayat Hani

مشرف أطروحة جامعية

al-Zaraini, Wail Ali Hasan
al-Tarawinah, Khalid Ahmad

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2011

الملخص الإنجليزي

Twenty bacterial isolates were obtained from different soil habitats in Al- Karak region.

They were screened for their antimicrobial activity against six test microorganisms (Staphylococcus aureus, Bacillus subtilis, Micrococcu luteus, Proteus vulgaris, Escherichia coli, Klebsilla pneumoniae).

Five isolates exhibited antimicrobial activity against at least one of the test microorganisms.

One of these isolates, 1A1, was highly active.

It caused an inhibition zones about 24-30 mm in diameter in agar diffusion test.

Optimization of the conditions for production of bioactive extract was performed.

The crude extract of the bacterial of interest was subjected to partial purification by column chromatography.

The bioactivity of the partially purified compounds was tested by agar diffusion assay and their minimum inhibition concentration (MIC) was determined.

Isolate 1A1 was identified as a distinct species in the genus Bacillus using morphological and biochemical test as well as 16S rDNA technology.

التخصصات الرئيسية

الأحياء

الموضوعات

• المضادات الحيوية

عدد الصفحات

38

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-302052