Isolation and molecular characterization of antibiotic producing bacteria from soil of al-Karak region and their potential as source of antimicrobial agents
Other Title(s)
عزل و دراسة الخصائص الجزيئية للبكتيريا المنتجة للمضادات الحيوية من التربة في منطقة الكرك و تأثيرها كمضادات بكتيرية
Dissertant
Thesis advisor
al-Zaraini, Wail Ali Hasan
al-Tarawinah, Khalid Ahmad
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2011
English Abstract
Twenty bacterial isolates were obtained from different soil habitats in Al- Karak region.
They were screened for their antimicrobial activity against six test microorganisms (Staphylococcus aureus, Bacillus subtilis, Micrococcu luteus, Proteus vulgaris, Escherichia coli, Klebsilla pneumoniae).
Five isolates exhibited antimicrobial activity against at least one of the test microorganisms.
One of these isolates, 1A1, was highly active.
It caused an inhibition zones about 24-30 mm in diameter in agar diffusion test.
Optimization of the conditions for production of bioactive extract was performed.
The crude extract of the bacterial of interest was subjected to partial purification by column chromatography.
The bioactivity of the partially purified compounds was tested by agar diffusion assay and their minimum inhibition concentration (MIC) was determined.
Isolate 1A1 was identified as a distinct species in the genus Bacillus using morphological and biochemical test as well as 16S rDNA technology.
Main Subjects
Topics
No. of Pages
38
Table of Contents
Table of contents.
Abstract.
Chapter one : Theoretical background.
Chapter two : Literature review.
Chapter three : Design and methodology.
Chapter four : Result and discussion.
References.
American Psychological Association (APA)
al-Tarawinah, Ayat Hani. (2011). Isolation and molecular characterization of antibiotic producing bacteria from soil of al-Karak region and their potential as source of antimicrobial agents. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-302052
Modern Language Association (MLA)
al-Tarawinah, Ayat Hani. Isolation and molecular characterization of antibiotic producing bacteria from soil of al-Karak region and their potential as source of antimicrobial agents. (Master's theses Theses and Dissertations Master). Mutah University. (2011).
https://search.emarefa.net/detail/BIM-302052
American Medical Association (AMA)
al-Tarawinah, Ayat Hani. (2011). Isolation and molecular characterization of antibiotic producing bacteria from soil of al-Karak region and their potential as source of antimicrobial agents. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-302052
Language
English
Data Type
Arab Theses
Record ID
BIM-302052
