CAT3, a new bioinformatics tool for prediction of Caspase -3 substrate cleavage site

مقدم أطروحة جامعية

Ayyash, Munif

مشرف أطروحة جامعية

Suwaylih, Khalid M.
Ashhab, Yaqub

الجامعة

جامعة بيرزيت

الكلية

كلية الهندسة و التكنولوجيا

القسم الأكاديمي

دائرة علم الحاسوب

دولة الجامعة

فلسطين (الضفة الغربية)

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2007

الملخص الإنجليزي

Caspases are responsible for all the morphological and biochemical changes that end in apoptosis.

Their apoptotic function is done in a cascade, which includes the cleavage of many different substrates.

Some of these substrates are converted from inactive form to active form through this cleavage while others are considered as controllers and inducers for reactions and processes included in apoptosis.

In general, caspases shared a remarkable feature in their cleavage process which is the specificity to Aspartic acid (D) in the P1 sub site.

All caspases substrates are cleaved after the amino acid “D”.

Caspase-3 (interleukin-1beta converting enzyme / CED-3) is the main executer caspase that is responsible for the cleavage of many key proteins.

Up-to-date ; caspase-3 has more than 150 known substrates experimentally.

A tool for predicting a substrate cleavage site / s becomes a need for most of researchers who work in apoptotic and cancer field and other related fields.

The few available bioinformatics tools have very low accuracy.

The present study introduces a new bioinformatics tool to predict the cleavage site of caspase-3 substrates.

CAT3 “Caspase-3 Tool” is specific only to caspase-3.

This specificity makes CAT3 a powerful tool with a higher accuracy compared to other available related tools.

This tool comes to predict the undetermined cleavage sites of many proteins defined as caspase-3 substrates.

Also it can predict other substrates that still not considered as a caspase-3 substrate.

CAT3 successfully predicts 23 out of 27 cleavage sites (about 85.2 %) of randomly chosen substrates that their cleavage sites are experimentally determined.

التخصصات الرئيسية

الأحياء
تكنولوجيا المعلومات وعلم الحاسوب

الموضوعات

• موت الخلية المبرمج

عدد الصفحات

120

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-303381