CAT3, a new bioinformatics tool for prediction of Caspase -3 substrate cleavage site
Dissertant
Thesis advisor
Suwaylih, Khalid M.
Ashhab, Yaqub
University
Birzeit University
Faculty
Faculty of Engineering and Technology
Department
Department of Computer Science
University Country
Palestine (West Bank)
Degree
Master
Degree Date
2007
English Abstract
Caspases are responsible for all the morphological and biochemical changes that end in apoptosis.
Their apoptotic function is done in a cascade, which includes the cleavage of many different substrates.
Some of these substrates are converted from inactive form to active form through this cleavage while others are considered as controllers and inducers for reactions and processes included in apoptosis.
In general, caspases shared a remarkable feature in their cleavage process which is the specificity to Aspartic acid (D) in the P1 sub site.
All caspases substrates are cleaved after the amino acid “D”.
Caspase-3 (interleukin-1beta converting enzyme / CED-3) is the main executer caspase that is responsible for the cleavage of many key proteins.
Up-to-date ; caspase-3 has more than 150 known substrates experimentally.
A tool for predicting a substrate cleavage site / s becomes a need for most of researchers who work in apoptotic and cancer field and other related fields.
The few available bioinformatics tools have very low accuracy.
The present study introduces a new bioinformatics tool to predict the cleavage site of caspase-3 substrates.
CAT3 “Caspase-3 Tool” is specific only to caspase-3.
This specificity makes CAT3 a powerful tool with a higher accuracy compared to other available related tools.
This tool comes to predict the undetermined cleavage sites of many proteins defined as caspase-3 substrates.
Also it can predict other substrates that still not considered as a caspase-3 substrate.
CAT3 successfully predicts 23 out of 27 cleavage sites (about 85.2 %) of randomly chosen substrates that their cleavage sites are experimentally determined.
Main Subjects
Biology
Information Technology and Computer Science
Topics
No. of Pages
120
Table of Contents
Table of contents.
Abstract
Abstract in Arabic.
Chapter one : Introduction.
Chapter two : Materials and methods.
Chapter three :
Chapter four : CAT3 algorithm.
Chapter five : Discussion and conclusions.
References.
American Psychological Association (APA)
Ayyash, Munif. (2007). CAT3, a new bioinformatics tool for prediction of Caspase -3 substrate cleavage site. (Master's theses Theses and Dissertations Master). Birzeit University, Palestine (West Bank)
https://search.emarefa.net/detail/BIM-303381
Modern Language Association (MLA)
Ayyash, Munif. CAT3, a new bioinformatics tool for prediction of Caspase -3 substrate cleavage site. (Master's theses Theses and Dissertations Master). Birzeit University. (2007).
https://search.emarefa.net/detail/BIM-303381
American Medical Association (AMA)
Ayyash, Munif. (2007). CAT3, a new bioinformatics tool for prediction of Caspase -3 substrate cleavage site. (Master's theses Theses and Dissertations Master). Birzeit University, Palestine (West Bank)
https://search.emarefa.net/detail/BIM-303381
Language
English
Data Type
Arab Theses
Record ID
BIM-303381
