تقييد أنزيم الألفا-اميليز المنتج من مالت الشعير المحلي بألجينات الكالسيوم

الملخص EN

The study included, Alpha-amylase immobilized which was produced from malt of local barley by four different binding materials.

The best one was calcium alginate, which gave highest binding for protein (9.25 mg / gm) and highest efficiency of enzyme immobilization 68 %.

Of Calcium alginate.

Best concentration of Calcium alginate to bind enzyme was 4 % which gave an enzyme activity of 160 unit / g and good hardens Volume of 2ml was chosen as the best volume of calcium alginate to bind alpha–amylase.

Increasing of binding enzyme from 0.5 -3 g was found to result in an showed a gradually increase in enzyme activity at a gradually and gave highest activity at a quantity of 3 g of enzyme binding calcium alginate.

Enzyme–binding calcium alginate was found to retain 98.5 % of alpha– amylase activity when using it 6 folds.

Effect of amount of enzyme–binding calcium alginate on starch solution viscosity was found to decrease gradually after 5 minutes from the binding of the reaction, the relative viscosity reached 70 % then 60 min from the start of the reaction.