Biodegradation of chloroaromatic compounds by bacteria isolated from as-Samra wastewater treatment plant

مقدم أطروحة جامعية

al-Rgibat, Iman Ahmad

مشرف أطروحة جامعية

Irshaid, Fawzi
al-Tarawinah, Khalid Ahmad

أعضاء اللجنة

Jaran, Adnan S.
Khalaf, Maruf A.
Khulayfat, Khalid Muhammad

الجامعة

جامعة آل البيت

الكلية

كلية العلوم

القسم الأكاديمي

قسم العلوم الحياتية

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2009

الملخص الإنجليزي

Citrobacter fundi was isolated from the As-Samra wastewater treatment plant, which is located in northern Jordan.

It was identified by using morphological and biochemical characteristics.

Citrobacter fundi were found to use the chlorobenzoate compounds as alternative carbon and energy source.

The percentages of degradation of these chlorobenzoate (2- chloro benzoic acid, 3- chloro benzoic acid, 4- chloro benzoic acid and 3,4- di chloro benzoic acid) at concentration 2-mM were found 70.4 %, 39.5 %, 38% and 61 % respectively, after 80 h of incubation.

At 3mM concentration the rates were 82%, 32 %, 49 % and 44.5 % respectively.

The initial rates were found different according to the substrate and its concentration.

The highest initial rates were obtained with the 3mM concentration for 2CBA.

The order of initial rates was 64.3µM / h (2- chloro benzoic acid), 50.7 µM / h (4- chloro benzoic acid), 42.2 µM / h (3, 4- di chloro benzoic acid) and 6.2 µM / h (3- chloro benzoic acid).

The biodegradation ability was monitored through the release of chloride, disappearance of the substrate and finally the growth of bacterial cells on that substrate.

The best results of degradation of 2-CBA compound were obtained with 3mM substrate concentration, 37ºC, pH 7and 100µl inoculums size.

Adaptation of the cells on 2-CBA for 48 hours and cells starvation for 24 hours and 48 hours increased slightly the initial degradation rate.

The carbon sources increased the cell biomass.

However, all carbon sources used reduced the biodegradation and Cl production from 2-CBA.

All glucose concentration enhanced the growth and reduced the degradation percentage as well as the Cl production.

Yeast extract and casein reduced the degradation activity of the 2-CBA as well as the chlorine release from it.

2-CBA (3mM) was used in further experiments because the 2-CBA is the most toxic one among these compounds.

The experiments included substrate concentration, temperature, pH, inoculum size, carbon starvation, carbon adaptation, and carbon and nitrogen sources.

The preferential concentration, temperature and pH for the strain were 3mM, 37ºC and 7 respectively.

التخصصات الرئيسية

علوم الأرض و المياه و البيئة

الموضوعات

• الصرف الصحي
• مياه الصرف الصحي
• الأردن

عدد الصفحات

104

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-307526